PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.

Trubitsyna, Maryia; Liu, Chao-Kuo; Salinas, Alejandro; Elfick, Alistair; French, Christopher E

Trubitsyna, Maryia; Liu, Chao-Kuo; Salinas, Alejandro; Elfick, Alistair; French, Christopher E.

Afiliación

Trubitsyna M; School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK. M.Trubitsyna@ed.ac.uk.
Liu CK; School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK.
Salinas A; School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK.
Elfick A; School of Engineering, University of Edinburgh, Faraday Building, Edinburgh, EH9 3JL, UK.
French CE; School of Biological Sciences, University of Edinburgh, Roger Land Building, Edinburgh, EH9 3FF, UK. C.French@ed.ac.uk.

Methods Mol Biol ; 1472: 111-28, 2017.

Article en En | MEDLINE | ID: mdl-27671936

RESUMEN

Joining DNA sequences to create linear and circular constructs is a basic requirement in molecular biology. Here we describe PaperClip, a recently developed method, which enables assembly of multiple DNA sequences in one reaction in a combinatorial manner. In contrast to other homology-based multi-part assembly methods currently available, PaperClip allows assembly of a given set of parts in any order without requiring specific single-use oligonucleotides for each assembly order.

Asunto(s)

ADN/síntesis química; Clonación Molecular; ADN Ligasas/química; Electroforesis en Gel de Agar; Escherichia coli/genética; Oligonucleótidos; Reacción en Cadena de la Polimerasa

Palabras clave

Agarose gel electrophoresis; CPEC; Cell extract; DNA assembly; Ligation; Molecular cloning; Oligonucleotide annealing; PAGE; PCR; SLiCE; Selection; Single-pot assembly

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google