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Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy.
Wakisaka, Yoshifumi; Suzuki, Yuta; Iwata, Osamu; Nakashima, Ayaka; Ito, Takuro; Hirose, Misa; Domon, Ryota; Sugawara, Mai; Tsumura, Norimichi; Watarai, Hiroshi; Shimobaba, Tomoyoshi; Suzuki, Kengo; Goda, Keisuke; Ozeki, Yasuyuki.
Afiliación
  • Wakisaka Y; Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
  • Suzuki Y; Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan.
  • Iwata O; Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
  • Nakashima A; Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
  • Ito T; Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
  • Hirose M; Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa 252-8520, Japan.
  • Domon R; Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
  • Sugawara M; Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
  • Tsumura N; Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
  • Watarai H; Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
  • Shimobaba T; Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
  • Suzuki K; Department of Electrical and Electronics Engineering, Chiba University, Chiba 263-8522, Japan.
  • Goda K; Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
  • Ozeki Y; Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
Nat Microbiol ; 1(10): 16124, 2016 08 01.
Article en En | MEDLINE | ID: mdl-27670110
Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría Raman / Agua / Euglena gracilis Límite: Animals Idioma: En Revista: Nat Microbiol Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría Raman / Agua / Euglena gracilis Límite: Animals Idioma: En Revista: Nat Microbiol Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido