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Analysis of SOX2-Regulated Transcriptome in Glioma Stem Cells.
Acanda de la Rocha, Arlet M; López-Bertoni, Hernando; Guruceaga, Elizabeth; González-Huarriz, Marisol; Martínez-Vélez, Naiara; Xipell, Enric; Fueyo, Juan; Gomez-Manzano, Candelaria; Alonso, Marta M.
Afiliación
  • Acanda de la Rocha AM; The Health Research Institute of Navarra (IDISNA), Pamplona, Spain.
  • López-Bertoni H; Program in Solid Tumors and Biomarkers, Foundation for the Applied Medical Research, Pamplona, Spain.
  • Guruceaga E; Department of Pediatrics, University Hospital of Navarra, Pamplona, Spain.
  • González-Huarriz M; Hugo W Moser Research Institute at Kennedy Krieger, Baltimore, Maryland, United States of America.
  • Martínez-Vélez N; Department of Neurology, The Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America.
  • Xipell E; The Health Research Institute of Navarra (IDISNA), Pamplona, Spain.
  • Fueyo J; Bioinformatics Unit, Center for Applied Medical Research, Pamplona, Spain.
  • Gomez-Manzano C; The Health Research Institute of Navarra (IDISNA), Pamplona, Spain.
  • Alonso MM; Program in Solid Tumors and Biomarkers, Foundation for the Applied Medical Research, Pamplona, Spain.
PLoS One ; 11(9): e0163155, 2016.
Article en En | MEDLINE | ID: mdl-27669421
INTRODUCTION: Glioblastoma is the most malignant brain tumor in adults and is associated with poor survival despite multimodal treatments. Glioma stem-like cells (GSCs) are cells functionally defined by their self-renewal potential and the ability to reconstitute the original tumor upon orthotopic implantation. They have been postulated to be the culprit of glioma chemo- and radio-resistance ultimately leading to relapse. Understanding the molecular circuits governing the GSC compartment is essential. SOX2, a critical transcription regulator of embryonic and neural stem cell function, is deregulated in GSCs however; the precise molecular pathways regulated by this gene in GSCs remain poorly understood. RESULTS: We performed a genome-wide analysis of SOX2-regulated transcripts in GSCs, using a microarray. We identified a total of 2048 differentially expressed coding transcripts and 261 non-coding transcripts. Cell adhesion and cell-cell signaling are among the most enriched terms using Gene Ontology (GO) classification. The pathways altered after SOX2 down-modulation includes multiple cellular processes such as amino-acid metabolism and intercellular signaling cascades. We also defined and classified the set of non-coding transcripts differentially expressed regulated by SOX2 in GSCs, and validated two of them. CONCLUSIONS: We present a comprehensive analysis of the transcriptome controlled by SOX2 in GSCs, gaining insights in the understanding of the potential roles of SOX2 in glioblastoma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos