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Opposite Function of ERα and ERß in Controlling 17ß-Estradiol-mediated Osteogenesis in Osteoblasts.
Wang, Yu-Xiang; Li, Min; Zhang, Hong-Qi; Tang, Ming-Xing; Guo, Chao-Feng; Deng, Ang; Chen, Yong; Xiao, Li-Ge.
Afiliación
  • Wang YX; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China.
  • Li M; Xiangya Stomatology Hospital of Central South University, ChangSha, Hunan, China.
  • Zhang HQ; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China. Electronic address: zhanghongqi9996@sina.com.
  • Tang MX; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China.
  • Guo CF; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China.
  • Deng A; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China.
  • Chen Y; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China.
  • Xiao LG; Department of Spine Surgery, Xiangya Spinal Surgery Center, Xiangya Hospital of Central South University, ChangSha, Hunan, China.
Arch Med Res ; 47(4): 255-61, 2016 05.
Article en En | MEDLINE | ID: mdl-27664484
Estrogen receptor plays critical roles in osteogenesis but the underlying mechanism remains unclear. In order to determine the effect of ERα and ERß on several critical factors in regulating osteogenesis in human osteoblasts. Cell based assy, RT-PCR and immunoblot analyses were used in the research. Both RT-PCR and immunoblot showed that gene expression of OPG, MBP2, TGF-ß, RUNX2, IGF-1 was significantly reduced while expression of RANKL was drastically increased after shRNA-based depletion of ERα in MG-63 osteoblasts. Surprisingly, 17ß-estradiol (E2) treatment led to remarkably reduced RANKL compared with that in E2 untreated cells. In contrast, ERß plays an opposite role in regulating gene expression of OPG, MBP2, TGF-ß, RUNX2, IGF-1 and RANKL. However, double depletion of ERα and ERß could not rescue the gene expression of these factors in vitro. Our results provide a novel mechanism of estrogen receptor in controlling osteogenesis in human cells as well as a potential clinic therapeutic target in human osteoporosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Receptor alfa de Estrógeno / Receptor beta de Estrógeno / Estradiol Límite: Humans Idioma: En Revista: Arch Med Res Asunto de la revista: MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoblastos / Receptor alfa de Estrógeno / Receptor beta de Estrógeno / Estradiol Límite: Humans Idioma: En Revista: Arch Med Res Asunto de la revista: MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos