Your browser doesn't support javascript.
loading
Leisingera sp. JC1, a Bacterial Isolate from Hawaiian Bobtail Squid Eggs, Produces Indigoidine and Differentially Inhibits Vibrios.
Gromek, Samantha M; Suria, Andrea M; Fullmer, Matthew S; Garcia, Jillian L; Gogarten, Johann Peter; Nyholm, Spencer V; Balunas, Marcy J.
Afiliación
  • Gromek SM; Division of Medicinal Chemistry, Department of Pharmaceutical Sciences, University of Connecticut Storrs, CT, USA.
  • Suria AM; Department of Molecular and Cell Biology, University of Connecticut Storrs, CT, USA.
  • Fullmer MS; Department of Molecular and Cell Biology, University of Connecticut Storrs, CT, USA.
  • Garcia JL; Division of Medicinal Chemistry, Department of Pharmaceutical Sciences, University of Connecticut Storrs, CT, USA.
  • Gogarten JP; Department of Molecular and Cell Biology, University of ConnecticutStorrs, CT, USA; Institute for Systems Genomics, University of ConnecticutStorrs, CT, USA.
  • Nyholm SV; Department of Molecular and Cell Biology, University of Connecticut Storrs, CT, USA.
  • Balunas MJ; Division of Medicinal Chemistry, Department of Pharmaceutical Sciences, University of Connecticut Storrs, CT, USA.
Front Microbiol ; 7: 1342, 2016.
Article en En | MEDLINE | ID: mdl-27660622
Female members of many cephalopod species house a bacterial consortium in the accessory nidamental gland (ANG), part of the reproductive system. These bacteria are deposited into eggs that are then laid in the environment where they must develop unprotected from predation, pathogens, and fouling. In this study, we characterized the genome and secondary metabolite production of Leisingera sp. JC1, a member of the roseobacter clade (Rhodobacteraceae) of Alphaproteobacteria isolated from the jelly coat of eggs from the Hawaiian bobtail squid, Euprymna scolopes. Whole genome sequencing and MLSA analysis revealed that Leisingera sp. JC1 falls within a group of roseobacters associated with squid ANGs. Genome and biochemical analyses revealed the potential for and production of a number of secondary metabolites, including siderophores and acyl-homoserine lactones involved with quorum sensing. The complete biosynthetic gene cluster for the pigment indigoidine was detected in the genome and mass spectrometry confirmed the production of this compound. Furthermore, we investigated the production of indigoidine under co-culture conditions with Vibrio fischeri, the light organ symbiont of E. scolopes, and with other vibrios. Finally, both Leisingera sp. JC1 and secondary metabolite extracts of this strain had differential antimicrobial activity against a number of marine vibrios, suggesting that Leisingera sp. JC1 may play a role in host defense against other marine bacteria either in the eggs and/or ANG. These data also suggest that indigoidine may be partially, but not wholly, responsible for the antimicrobial activity of this squid-associated bacterium.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza