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N-glycosylation enables high lateral mobility of GPI-anchored proteins at a molecular crowding threshold.
Hartel, Andreas J W; Glogger, Marius; Jones, Nicola G; Abuillan, Wasim; Batram, Christopher; Hermann, Anne; Fenz, Susanne F; Tanaka, Motomu; Engstler, Markus.
Afiliación
  • Hartel AJ; Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg 97074, Germany.
  • Glogger M; Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg 97074, Germany.
  • Jones NG; Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg 97074, Germany.
  • Abuillan W; Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg, Heidelberg 69120, Germany.
  • Batram C; Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg 97074, Germany.
  • Hermann A; Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg 97074, Germany.
  • Fenz SF; Department of Cell and Developmental Biology, Theodor-Boveri-Institute, Biocenter, University of Würzburg, Würzburg 97074, Germany.
  • Tanaka M; Physical Chemistry of Biosystems, Institute of Physical Chemistry, University of Heidelberg, Heidelberg 69120, Germany.
  • Engstler M; Institute for Integrated Cell-Material Science (WPI iCeMS), Kyoto University, Kyoto 606-8501, Japan.
Nat Commun ; 7: 12870, 2016 09 19.
Article en En | MEDLINE | ID: mdl-27641538
The protein density in biological membranes can be extraordinarily high, but the impact of molecular crowding on the diffusion of membrane proteins has not been studied systematically in a natural system. The diversity of the membrane proteome of most cells may preclude systematic studies. African trypanosomes, however, feature a uniform surface coat that is dominated by a single type of variant surface glycoprotein (VSG). Here we study the density-dependence of the diffusion of different glycosylphosphatidylinositol-anchored VSG-types on living cells and in artificial membranes. Our results suggest that a specific molecular crowding threshold (MCT) limits diffusion and hence affects protein function. Obstacles in the form of heterologous proteins compromise the diffusion coefficient and the MCT. The trypanosome VSG-coat operates very close to its MCT. Importantly, our experiments show that N-linked glycans act as molecular insulators that reduce retarding intermolecular interactions allowing membrane proteins to function correctly even when densely packed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas Variantes de Superficie de Trypanosoma Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas Variantes de Superficie de Trypanosoma Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido