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The Formation of Blood Vessel After the Administration of the Plasmid Encoding Ang-1 Gene in Fischer Rats.
Skóra, Jan P; Plonek, Tomasz; Barc, Piotr; Baczynska, Dagmara; Radwanska, Agata; Pupka, Artur; Korta, Krzysztof; Ussowicz, Marek; Strozecki, Lukasz; Kupczynska-Markiewicz, Diana; Halon, Agnieszka.
Afiliación
  • Skóra JP; Department of Vascular, General and Transplantation Surgery, Wroclaw Medical University, Poland.
  • Plonek T; Department of Cardiac Surgery, Wroclaw Medical University, Poland.
  • Barc P; Department of Vascular, General and Transplantation Surgery, Wroclaw Medical University, Poland.
  • Baczynska D; Department of Forensic Medicine, Molecular Techniques Unit, Wroclaw Medical University, Poland.
  • Radwanska A; Wrovasc - Integrated Cardiovascular Centre, Regional Specialist Hospital in Wroclaw, Research and Development Centre, Poland.
  • Pupka A; Wrovasc - Integrated Cardiovascular Centre, Regional Specialist Hospital in Wroclaw, Research and Development Centre, Poland.
  • Korta K; Department of Vascular, General and Transplantation Surgery, Wroclaw Medical University, Poland.
  • Ussowicz M; Department of Vascular, General and Transplantation Surgery, Wroclaw Medical University, Poland.
  • Strozecki L; Department of Pediatric BMT, Oncology and Hematology, Wroclaw Medical University, Poland.
  • Kupczynska-Markiewicz D; Department of Anaesthesiology and Intensive Therapy, Wroclaw Medical University, Poland.
  • Halon A; Department of Vascular, General and Transplantation Surgery, Wroclaw Medical University, Poland.
Adv Clin Exp Med ; 25(4): 611-5, 2016.
Article en En | MEDLINE | ID: mdl-27629833
BACKGROUND: Chronic limb ischemia is a serious clinical problem. Patients who do not qualify for standard treatment may benefit from novel gene therapies. OBJECTIVES: This study evaluated angiogenesis following intramuscular injections of angiogenic plasmid Ang-1 in Fisher rats. MATERIAL AND METHODS: Twenty rats had plasmids injected intramuscularly in their hind limbs. The study group consisted of 10 animals which received the Ang-1 plasmid, while the control group consisted of 10 rats that received an empty plasmid. All the animals were euthanized after 12 weeks and tissue samples from the hind limb thigh muscles and internal organs were harvested for histological and immunohistochemical examinations. To assess the angiogenesis the number of vessels in the hind limb muscles visualized by the SMA and FVIII markers was counted for each animal in five separate microscopic fields. RESULTS: There were no pathological lesions or any signs of neoplastic angiogenesis in any of the 20 rats. The number of vessels visualized by the FVIII marker in the study group was two times higher than in the control group (median: 12, range: 7-25 vs. median: 6, range: 2-15; p < 0.0001). The median estimated that the number of vessels visualized by the SMA marker is 63% higher in the study group compared to the control group (median: 6.5, range: 1-12 vs. median: 4, range: 0-10; p = 0.0008). CONCLUSIONS: Intramuscular injections of Ang-1 plasmids induced angiogenesis in the rat hind limb muscles.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neovascularización Fisiológica / Angiopoyetina 1 Límite: Animals Idioma: En Revista: Adv Clin Exp Med Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Polonia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neovascularización Fisiológica / Angiopoyetina 1 Límite: Animals Idioma: En Revista: Adv Clin Exp Med Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Polonia