Transient fusion ensures granule replenishment to enable repeated release after IgE-mediated mast cell degranulation.
J Cell Sci
; 129(21): 3989-4000, 2016 11 01.
Article
en En
| MEDLINE
| ID: mdl-27624612
To ensure normal immune function, mast cells employ different pathways to release mediators. Here, we report a thus far unknown capacity of mast cells to recycle and reuse secretory granules after an antigen-evoked degranulation process under physiological conditions; this phenomenon involves the existence of a recycling secretory granule pool that is available for release in a short time scale. Rapid endocytic modes contributed to the recycling of â¼60% of the total secretory granule population, which involved kiss-and-run and cavicapture mechanisms, causing retention of the intragranular matrix. We found the presence of normal-size granules and giant actomyosin- and dynamin-dependent granules, which were characterized by large quantal content. These large structures allowed the recovered mast cells to release a large amount of 5-HT, compensating for the decrease in the number of exocytosed secretory granules. This work uncovers a new physiological role of the exo-endocytosis cycle in the immunological plasticity of mast cells and reveals a new property of their biological secretion.
Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Inmunoglobulina E
/
Degranulación de la Célula
/
Vesículas Secretoras
/
Mastocitos
/
Fusión de Membrana
Límite:
Animals
Idioma:
En
Revista:
J Cell Sci
Año:
2016
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Reino Unido