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Restriction site detection in repetitive nuclear DNA sequences of Trypanosoma evansi for strain differentiation among different isolates.
Shyma, K P; Gupta, S K; Gupta, J P; Singh, Ajit; Chaudhari, S S; Singh, Veer.
Afiliación
  • Shyma KP; Department of Parasitology, College of Veterinary Sciences & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
  • Gupta SK; Departments of Veterinary Parasitology, College of Veterinary Science, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar, 125004 Haryana India.
  • Gupta JP; Department of AGB, College of Veterinary Sciences & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
  • Singh A; Department of Immunology, College of Veterinary Science, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar, 125004 Haryana India.
  • Chaudhari SS; Department of TVCC, College of Veterinary Science, Lala Lajapat Rai University of Veterinary and Animal Sciences, Hisar, 125004 Haryana India.
  • Singh V; Department of Parasitology, College of Veterinary Sciences & Animal Husbandry, Sardarkrushinagar Dantiwada Agricultural University, Gujarat, India.
J Parasit Dis ; 40(3): 1087-90, 2016 Sep.
Article en En | MEDLINE | ID: mdl-27605842
The differences or similarities among different isolates of Trypanosoma evansi through endonuclease profile was identified in the present study. The repetitive nuclear DNA of T. evansi isolated from infected cattle, buffalo and equine blood was initially amplified by PCR using specific primers. A panel of restriction enzymes, EcoRI, Eco91l, HindIII and PstI were for complete digestion of PCR products. Agarose gel electrophoresis of digested product did not show cleavage fragments and only single DNA band of the original size was visible in the ethidium bromide stained agarose gel. This indicated that the 227 bp PCR product from repetitive sequence had no site-specific cleavage sites for the REs used in this study. No heterogeneity in the repetitive nuclear DNA restriction endonuclease profile among the different isolates was recorded.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Parasit Dis Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: J Parasit Dis Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: India