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A Subset of Neutrophils Expressing Markers of Antigen-Presenting Cells in Human Visceral Leishmaniasis.
Sharma, Smriti; Davis, Richard E; Srivastva, Shweta; Nylén, Susanne; Sundar, Shyam; Wilson, Mary E.
Afiliación
  • Sharma S; Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
  • Davis RE; Interdisciplinary Program in Immunology, University of Iowa.
  • Srivastva S; Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
  • Nylén S; Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
  • Sundar S; Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
  • Wilson ME; Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
J Infect Dis ; 214(10): 1531-1538, 2016 11 15.
Article en En | MEDLINE | ID: mdl-27601622
BACKGROUND: Visceral leishmaniasis (VL) is a potentially fatal parasitic disease associated with fever, cachexia and impaired protective T-cell responses against the parasite. METHODS: Peripheral blood leukocytes from 105 subjects with VL and healthy control subjects from the endemic region of Muzaffarpur, Bihar, India, were compared using flow cytometry and reverse-transcriptase quantitative polymerase chain reaction. Findings were correlated with clinical data. RESULTS: An expanded population of low-density neutrophils that expressed HLA-DR, CD80 and CD86 was observed in subjects with VL. This neutrophil population contracted after successful treatment of disease. Plasma from patients with acute VL was able to induce similar high-level HLA-DR expression in neutrophils from healthy subjects. HLA-DR+ neutrophils from subjects with VL did not stimulate T-cell proliferation, but they did express higher programmed cell death ligand-1 (PDL1) than other neutrophils, and lymphocytes of the same subjects expressed high programmed cell death 1 (PD1). CONCLUSIONS: Patients with acute VL have expanded circulating low-density neutrophils expressing markers of antigen presentation, which diminish after treatment. Development of HLA-DR+ neutrophils is stimulated, at least in part, by components of plasma from patients with acute disease. Although we found no evidence that they act as antigen-presenting cells, these neutrophils expressed markers implicating a role in T-cell exhaustion.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmaniasis Visceral / Células Presentadoras de Antígenos / Neutrófilos Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged País/Región como asunto: Asia Idioma: En Revista: J Infect Dis Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Leishmaniasis Visceral / Células Presentadoras de Antígenos / Neutrófilos Límite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged País/Región como asunto: Asia Idioma: En Revista: J Infect Dis Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos