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High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays.
Cermak, Nathan; Olcum, Selim; Delgado, Francisco Feijó; Wasserman, Steven C; Payer, Kristofor R; A Murakami, Mark; Knudsen, Scott M; Kimmerling, Robert J; Stevens, Mark M; Kikuchi, Yuki; Sandikci, Arzu; Ogawa, Masaaki; Agache, Vincent; Baléras, François; Weinstock, David M; Manalis, Scott R.
Afiliación
  • Cermak N; Program in Computational and Systems Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Olcum S; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Delgado FF; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Wasserman SC; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Payer KR; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • A Murakami M; Microsystems Technology Laboratories, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Knudsen SM; Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA.
  • Kimmerling RJ; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Stevens MM; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Kikuchi Y; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Sandikci A; Microsystems Technology Laboratories, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Ogawa M; Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
  • Agache V; Innovative Micro Technology, Goleta, California, USA.
  • Baléras F; CEA-LETI, Minatec Campus, Grenoble, France.
  • Weinstock DM; CEA-LETI, Minatec Campus, Grenoble, France.
  • Manalis SR; Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts, USA.
Nat Biotechnol ; 34(10): 1052-1059, 2016 Oct.
Article en En | MEDLINE | ID: mdl-27598230
Methods to rapidly assess cell growth would be useful for many applications, including drug susceptibility testing, but current technologies have limited sensitivity or throughput. Here we present an approach to precisely and rapidly measure growth rates of many individual cells simultaneously. We flow cells in suspension through a microfluidic channel with 10-12 resonant mass sensors distributed along its length, weighing each cell repeatedly over the 4-20 min it spends in the channel. Because multiple cells traverse the channel at the same time, we obtain growth rates for >60 cells/h with a resolution of 0.2 pg/h for mammalian cells and 0.02 pg/h for bacteria. We measure the growth of single lymphocytic cells, mouse and human T cells, primary human leukemia cells, yeast, Escherichia coli and Enterococcus faecalis. Our system reveals subpopulations of cells with divergent growth kinetics and enables assessment of cellular responses to antibiotics and antimicrobial peptides within minutes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proliferación Celular / Evaluación Preclínica de Medicamentos / Sistemas Microelectromecánicos / Ensayos Analíticos de Alto Rendimiento / Dispositivos Laboratorio en un Chip Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proliferación Celular / Evaluación Preclínica de Medicamentos / Sistemas Microelectromecánicos / Ensayos Analíticos de Alto Rendimiento / Dispositivos Laboratorio en un Chip Tipo de estudio: Diagnostic_studies / Evaluation_studies Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos