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Comparison of agar dilution and broth microdilution methods for Clostridium difficile antimicrobial susceptibility testing.
Igawa, Gene; Casey, Mika; Sawabe, Etsuko; Nukui, Yoko; Okugawa, Shu; Moriya, Kyoji; Koike, Ryuji; Tohda, Shuji; Saito, Ryoichi.
Afiliación
  • Igawa G; Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
  • Casey M; Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
  • Sawabe E; Department of Clinical Laboratory, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan; Department of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan.
  • Nukui Y; Department of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan.
  • Okugawa S; Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan.
  • Moriya K; Department of Infection Control and Prevention, The University of Tokyo Hospital, Tokyo, Japan.
  • Koike R; Department of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan.
  • Tohda S; Department of Clinical Laboratory, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan.
  • Saito R; Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan; Department of Infection Control and Prevention, Medical Hospital, Tokyo Medical and Dental University, Tokyo, Japan. Electronic address: r-saito.mi@tmd.ac.jp.
J Glob Antimicrob Resist ; 7: 43-45, 2016 Dec.
Article en En | MEDLINE | ID: mdl-27598055
In this study, the performance of the broth microdilution (BMD) method for testing the antimicrobial susceptibility of Clostridium difficile in comparison with the agar dilution (AD) method used by the Clinical and Laboratory Standards Institute (CLSI) was evaluated. In total, 70 non-duplicate C. difficile clinical isolates were used in this study. The minimum inhibitory concentrations (MICs) of clindamycin, moxifloxacin, metronidazole and vancomycin were examined using AD and BMD. The results showed that BMD is acceptable for routine antimicrobial susceptibility testing of C. difficile as its performance was comparable with that of the AD method. In addition, it was noted that metronidazole- and vancomycin-resistant isolates are extremely rare in Japan.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Clostridioides difficile / Antibacterianos Tipo de estudio: Guideline País/Región como asunto: Asia Idioma: En Revista: J Glob Antimicrob Resist Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas de Sensibilidad Microbiana / Clostridioides difficile / Antibacterianos Tipo de estudio: Guideline País/Región como asunto: Asia Idioma: En Revista: J Glob Antimicrob Resist Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos