Incidence of apoptosis after retinoids and insulin-like growth factor-I (IGF-I) supplementation during goat in vitro embryo production.
Zygote
; 24(6): 808-813, 2016 Dec.
Article
en En
| MEDLINE
| ID: mdl-27587268
The addition of growth factors and vitamins enhances goat embryonic development in vitro. However, few attempts have been reported trying to identify supplementation regimens for oocyte maturation or embryo culture with additive properties. The present report was aimed to evaluate if retinoids [0.3 µM retinyl acetate (RAc) and 0.5 µM 9-cis-retinoic acid (RA)] supplementation during goat oocyte maturation and retinoids and/or 50 ng mL-1 IGF-I during embryo culture synergically enhanced embryonic development while diminishing the incidence of apoptosis. All combinations of RAc and RA treatment produced blastocysts with similar efficiencies, while IGF-I enhanced embryos yields irrespectively of retinoid addition. Moreover, retinoids and IGF-I supplementation showed similar caspase activity or DNA fragmentation indexes in blastocysts. In conclusion, supplementation with retinoids and IGF-I during goat embryo culture enhances blastocysts development without synergic reduction of apoptosis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Retinoides
/
Factor I del Crecimiento Similar a la Insulina
/
Apoptosis
/
Técnicas de Cultivo de Embriones
/
Técnicas de Maduración In Vitro de los Oocitos
Tipo de estudio:
Incidence_studies
/
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
Zygote
Asunto de la revista:
EMBRIOLOGIA
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Reino Unido