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Prunella vulgaris Polysaccharide Inhibits Growth and Migration of Breast Carcinoma-Associated Fibroblasts by Suppressing Expression of Basic Fibroblast Growth Factor.
Hao, Jian; Ding, Xiu-Li; Yang, Xue; Wu, Xiong-Zhi.
Afiliación
  • Hao J; Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
  • Ding XL; Clincal Center of Tumor Therapy, The Fourth Central Hospital Affiliated to Nankai University, Tianjin, 300140, China.
  • Yang X; Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
  • Wu XZ; Zhong-Shan-Men In-Patient Department, Tianjin Medical University Cancer Institute and Hospital; National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy, Tianjin, 300060, China.
Chin J Integr Med ; 26(4): 270-276, 2020 Apr.
Article en En | MEDLINE | ID: mdl-27586473
OBJECTIVE: To study the effects of Prunella vulgaris polysaccharide (PVP) on human breast carcinoma-associated fibroblasts (CAFs). METHODS: Cell viability was detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-(3-carboxymethoxyphenyl)-2-4-sulfophenyl)-2H-tetrazolium (MTS) assay. Wound healing experiment and transwell migration assay were used to investigate the anti-migration effects. Flow cytometry was applied to detect cell apoptosis and cell cycle distribution. Reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay were used to detect the expression of basic fibroblast growth factor (bFGF) in CAFs. Culture SKBr-3 with CAFs conditioned medium (CAFs-CM) to evaluate the indirect function on the proliferation of breast cancer SKBr-3 cells. RESULTS: PVP inhibited the viability of CAFs by inducing apoptosis (P <0.01) and arresting cell cycle (P <0.01). It also inhibited the migration of CAFs (P <0.01). bFGF promoted CAFs proliferation (P <0.01) and migration (P <0.01), protected CAFs from apoptosis (P <0.05) and reduced G0 phase to 49.06% (P <0.01). However, these effects of bFGF on CAFs could be abrogated by PVP. Culturing SKBr-3 with CAFs-CM, PVP could inhibit the viability of breast cancer SKBr-3 cells indirectly. Moreover, PVP reduced the mRNA expression (P <0.01) and protein secretion of bFGF (P <0.01) in CAFs. CONCLUSION: PVP could exert an anti-cancer effect on breast CAFs by inhibiting bFGF expression, thus inhibiting the growth of breast cancer SKBr-3 cells indirectly.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Neoplasias de la Mama / Extractos Vegetales / Factor 2 de Crecimiento de Fibroblastos / Prunella / Fibroblastos Tipo de estudio: Risk_factors_studies Límite: Female / Humans Idioma: En Revista: Chin J Integr Med Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Neoplasias de la Mama / Extractos Vegetales / Factor 2 de Crecimiento de Fibroblastos / Prunella / Fibroblastos Tipo de estudio: Risk_factors_studies Límite: Female / Humans Idioma: En Revista: Chin J Integr Med Asunto de la revista: TERAPIAS COMPLEMENTARES Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: China