Non-invasive, quantitative assessment of the morphology of Î³-irradiated human mesenchymal stem cells and periosteal cells using digital holographic microscopy.

Kawase, Tomoyuki; Okuda, Kazuhiro; Nagata, Masaki; Tsuchimochi, Makoto; Yoshie, Hiromasa; Nakata, Koh

Kawase, Tomoyuki; Okuda, Kazuhiro; Nagata, Masaki; Tsuchimochi, Makoto; Yoshie, Hiromasa; Nakata, Koh.

Afiliación

Kawase T; a Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.
Okuda K; b Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.
Nagata M; c Division of Oral and Maxillofacial Surgery , Institute of Medicine and Dentistry, Niigata University , Niigata , Japan.
Tsuchimochi M; d Department of Oral and Maxillofacial Radiology , The Nippon Dental University School of Life Dentistry at Niigata , Niigata , Japan.
Yoshie H; b Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata, Japan.
Nakata K; e Bioscience Medical Research Center, Niigata University Medical and Dental Hospital , Niigata , Japan.

Int J Radiat Biol ; 92(12): 796-805, 2016 12.

Article en En | MEDLINE | ID: mdl-27586164

RESUMEN

PURPOSE: To assure the quality of cells to be used in cell therapy, we examined the applicability of digital holographic microscopy (DHM) for non-invasive, quantitative assessment of changes in cell morphology. MATERIALS AND METHODS: Mesenchymal stem cells derived from adipose tissue (MSC-AT) and bone marrow (MSC-BM), in addition to human alveolar periosteal cells (PC) as a reference, were Î³-ray irradiated (1 and 4 Gy), and their morphological changes were quantified without fixation using holographic microscopy. After detachment and fixation with ethanol, cell number and surface antigen expression were determined using an automated cell counter kit and flow-cytometry, respectively. RESULTS: Among various indexes, only indexes related to cell size were significantly changed after Î³-irradiation. Both BMC-AT and BMC-BM were enlarged and more sensitive to a low dose of Î³-irradiation than PC. In contrast to PC, proteins related to DNA damage repair (Î³-H2AX, p21waf1, p53 and Rb) were not substantially upregulated or sustained for a week in either MSC-AT or MSC-BM. CONCLUSION: Instead of DNA damage markers, we suggest that cell morphological parameters (e.g. cell volume) that are monitored by DHM could be a useful and more stable marker of MSC quality.

Asunto(s)

Holografía/métodos; Células Madre Mesenquimatosas/citología; Células Madre Mesenquimatosas/efectos de la radiación; Microscopía/métodos; Periostio/citología; Periostio/efectos de la radiación; Tamaño de la Célula/efectos de la radiación; Rastreo Celular/métodos; Células Cultivadas; Relación Dosis-Respuesta en la Radiación; Rayos gamma; Humanos; Imagenología Tridimensional/métodos; Dosis de Radiación; Reproducibilidad de los Resultados; Sensibilidad y Especificidad; Procesamiento de Señales Asistido por Computador

Palabras clave

Cell volume; holographic microscopy; mesenchymal stem cells; periosteal cells; Î³-ray irradiation

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Holografía / Células Madre Mesenquimatosas / Microscopía Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: Int J Radiat Biol Asunto de la revista: RADIOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Reino Unido

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