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Wnt/ß-catenin signaling plays an important role in the protective effects of FDP-Sr against oxidative stress induced apoptosis in MC3T3-E1 cell.
Qi, Huan-Huan; Bao, Jun; Zhang, Qi; Ma, Bo; Gu, Gui-Ying; Zhang, Peng-Ling; Ou-Yang, Gang; Wu, Zi-Mei; Ying, Han-Jie; Ou-Yang, Ping-Kai.
Afiliación
  • Qi HH; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China.
  • Bao J; Jiangsu Province of Ficial Hospital, Nanjing 210024, People's Republic of China.
  • Zhang Q; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China. Electronic address: nancyzhang03@hotmail.com.
  • Ma B; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China. Electronic address: mabo19821221@163.com.
  • Gu GY; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 210009, People's Republic of China.
  • Zhang PL; Jiangsu Province of Ficial Hospital, Nanjing 210024, People's Republic of China.
  • Ou-Yang G; Jiangsu Province of Ficial Hospital, Nanjing 210024, People's Republic of China.
  • Wu ZM; School of Pharmacy, FMHS, The University of Auckland Private Bag 92019, Auckland 1142, New Zealand.
  • Ying HJ; School of Life Science & Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, People's Republic of China.
  • Ou-Yang PK; School of Life Science & Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, People's Republic of China.
Bioorg Med Chem Lett ; 26(19): 4720-4723, 2016 10 01.
Article en En | MEDLINE | ID: mdl-27575480
Strontium fructose 1,6-diphosphate (FDP-Sr) is a new strontium-containing compound. The primary aim of this study was to clarify whether the structure component of FDP-Sr, FDP could benefit the protective effect of Sr (II) against oxidative stress induced apoptosis, and meanwhile to further explore the important role of Wnt/ß-catenin signaling in the anti-apoptosis effect of FDP-Sr in response to oxidative stress induced by H2O2 in an osteoblastic MC3T3-E1 cell line. Results showed that FDP-Sr could improve the osteoblastic differentiation under oxidative stress with induced cell proliferation and improved mineralization. The inhibition effect of FDP-Sr on cell apoptosis induced by H2O2 was proved by reduced reactive oxygen species production and activated caspase3. Under oxidative stress, mRNA and protein levels of phospho-ß-catenin reduced, while ß-catenin increased in the FDP-Sr treatment cell, leaded to the up-regulations of Runx2 and OPG at both mRNA and protein levels, finally improved the differentiation of osteoblasts. By the engagement of Wnt/ß-catenin pathway's inhibitor (XAV-939), the protective effects of FDP-Sr on osteoblastic differentiation against oxidative stress were repressed along with inhibited wnt/ß-catenin signaling and reduced mRNA and protein levels of Runx2 and OPG. In conclusion, FDP-Sr was demonstrated to protect osteoblast differentiation from oxidative damage induced by H2O2 through up-regulation of Wnt/ß-catenin signaling, and FDP in FDP-Sr was able to directly improve the oxidative stress injury through its ROS scavenging ability.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleótidos / Formicinas / Transducción de Señal / Estrés Oxidativo / Proteínas Wnt Límite: Animals Idioma: En Revista: Bioorg Med Chem Lett Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleótidos / Formicinas / Transducción de Señal / Estrés Oxidativo / Proteínas Wnt Límite: Animals Idioma: En Revista: Bioorg Med Chem Lett Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido