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Laminin modification subretinal bio-scaffold remodels retinal pigment epithelium-driven microenvironment in vitro and in vivo.
Peng, Chi-Hsien; Chuang, Jen-Hua; Wang, Mong-Lien; Jhan, Yong-Yu; Chien, Ke-Hung; Chung, Yu-Chien; Hung, Kuo-Hsuan; Chang, Chia-Ching; Lee, Chao-Kuei; Tseng, Wei-Lien; Hwang, De-Kuang; Hsu, Chia-Hsien; Lin, Tai-Chi; Chiou, Shih-Hwa; Chen, Shih-Jen.
Afiliación
  • Peng CH; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Chuang JH; Department of Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital & Fu-Jen Catholic University, Taipei Taiwan.
  • Wang ML; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Jhan YY; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Chien KH; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Chung YC; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Hung KH; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Chang CC; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Lee CK; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Tseng WL; Department of Ophthalmology, Tri-Service General Hospital & National Defense Medical Center, Taipei, Taiwan.
  • Hwang DK; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Hsu CH; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Lin TC; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Chiou SH; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Chen SJ; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
Oncotarget ; 7(40): 64631-64648, 2016 Oct 04.
Article en En | MEDLINE | ID: mdl-27564261
Advanced age-related macular degeneration (AMD) may lead to geographic atrophy or fibrovascular scar at macular, dysfunctional retinal microenvironment, and cause profound visual loss. Recent clinical trials have implied the potential application of pluripotent cell-differentiated retinal pigment epithelial cells (dRPEs) and membranous scaffolds implantation in repairing the degenerated retina in AMD. However, the efficacy of implanted membrane in immobilization and supporting the viability and functions of dRPEs, as well as maintaining the retinal microenvironment is still unclear. Herein we generated a biomimetic scaffold mimicking subretinal Bruch's basement from plasma modified polydimethylsiloxane (PDMS) sheet with laminin coating (PDMS-PmL), and investigated its potential functions to provide a subretinal environment for dRPE-monolayer grown on it. Firstly, compared to non-modified PDMS, PDMS-PmL enhanced the attachment, proliferation, polarization, and maturation of dRPEs. Second, PDMS-PmL increased the polarized tight junction, PEDF secretion, melanosome pigment deposit, and phagocytotic-ability of dRPEs. Third, PDMS-PmL was able to carry a dRPEs/photoreceptor-precursors multilayer retina tissue. Finally, the in vivo subretinal implantation of PDMS-PmL in porcine eyes showed well-biocompatibility up to 2-year follow-up. Notably, multifocal ERGs at 2-year follow-up revealed well preservation of macular function in PDMS-PmL, but not PDMS, transplanted porcine eyes. Trophic PEDF secretion of macular retina in PDMS-PmL group was also maintained to preserve retinal microenvironment in PDMS-PmL eyes at 2 year. Taken together, these data indicated that PDMS-PmL is able to sustain the physiological morphology and functions of polarized RPE monolayer, suggesting its potential of rescuing macular degeneration in vivo.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Laminina / Células Madre Pluripotentes / Trasplante de Células Madre / Materiales Biomiméticos / Dimetilpolisiloxanos / Andamios del Tejido / Epitelio Pigmentado de la Retina / Degeneración Macular / Nylons Límite: Animals Idioma: En Revista: Oncotarget Año: 2016 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Laminina / Células Madre Pluripotentes / Trasplante de Células Madre / Materiales Biomiméticos / Dimetilpolisiloxanos / Andamios del Tejido / Epitelio Pigmentado de la Retina / Degeneración Macular / Nylons Límite: Animals Idioma: En Revista: Oncotarget Año: 2016 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Estados Unidos