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Long-term storage does not impact the quality of cryopreserved human ovarian tissue.
Fabbri, Raffaella; Macciocca, Maria; Vicenti, Rossella; Pasquinelli, Gianandrea; Caprara, Giacomo; Valente, Sabrina; Seracchioli, Renato; Paradisi, Roberto.
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  • Fabbri R; Gynecology and Physiopathology of Human Reproductive Unit, Department of Medical and Surgical Sciences, University of Bologna, S. Orsola-Malpighi Hospital of Bologna, via Massarenti 13, 40138, Bologna, Italy.
  • Macciocca M; Gynecology and Physiopathology of Human Reproductive Unit, Department of Medical and Surgical Sciences, University of Bologna, S. Orsola-Malpighi Hospital of Bologna, via Massarenti 13, 40138, Bologna, Italy.
  • Vicenti R; Gynecology and Physiopathology of Human Reproductive Unit, Department of Medical and Surgical Sciences, University of Bologna, S. Orsola-Malpighi Hospital of Bologna, via Massarenti 13, 40138, Bologna, Italy. rossella.vicenti@unibo.it.
  • Pasquinelli G; Surgical Pathology, Department of Experimental, Diagnostic and Speciality Medicine, University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy.
  • Caprara G; Histopathological and Molecular Diagnostic Unit of Solid Organ and Transplant, S. Orsola-Malpighi Hospital, Bologna, Italy.
  • Valente S; Surgical Pathology, Department of Experimental, Diagnostic and Speciality Medicine, University of Bologna, S. Orsola-Malpighi Hospital, Bologna, Italy.
  • Seracchioli R; Gynecology and Physiopathology of Human Reproductive Unit, Department of Medical and Surgical Sciences, University of Bologna, S. Orsola-Malpighi Hospital of Bologna, via Massarenti 13, 40138, Bologna, Italy.
  • Paradisi R; Gynecology and Physiopathology of Human Reproductive Unit, Department of Medical and Surgical Sciences, University of Bologna, S. Orsola-Malpighi Hospital of Bologna, via Massarenti 13, 40138, Bologna, Italy.
J Ovarian Res ; 9(1): 50, 2016 Aug 24.
Article en En | MEDLINE | ID: mdl-27557782
BACKGROUND: Ovarian tissue cryopreservation is an emerging technique, also addressed to very young cancer patients, for whom it is not possible to perform an ovarian stimulation for oocytes freezing, before gonadotoxic treatment. In this cases, ovarian tissue must be cryopreserved for a long period of time and it is very important to know if it maintains fertility function after a long period of storage. Here we aimed to assess the effect of long-term storage on preservation and viability of cryopreserved human ovarian tissue. METHODS: Descriptive study of three cases of cancer patients whose cryopreserved ovarian tissue remained stored for 18 years. Long-term stored tissue was examined by histological and immunohistochemical analysis, transmission electron microscopy, TUNEL assay and LIVE/DEAD viability/citotoxicity test. RESULTS: Ovarian tissue stored for 18 years showed a good morphology. Follicles presented negative staining for estrogen and progesterone receptors, positive staining for ki67 in granulosa cells and/or oocytes and for bcl2 in granulosa cells. Regarding stroma, patch/focal positive expression was found for estrogen receptor and ki67, diffusely positive expression for progesterone receptor and bcl2. After long-term storage, ultrastructural examination showed sub-cellular integrity of follicles and interstitial oedema foci. No apoptosis was observable by TUNEL assay. Stromal cell viability remained >97 % during the culture period. CONCLUSION: The evaluation of different aspects of the tissue provides evidence that the storage time does not impact on tissue quality and gives hope especially to cancer girls, whose tissues could remain cryopreserved for a very long time.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Criopreservación Límite: Adult / Female / Humans Idioma: En Revista: J Ovarian Res Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Criopreservación Límite: Adult / Female / Humans Idioma: En Revista: J Ovarian Res Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido