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Identification of a capsular variant and characterization of capsular acetylation in Klebsiella pneumoniae PLA-associated type K57.
Hsu, Chun-Ru; Liao, Chun-Hsing; Lin, Tzu-Lung; Yang, Han-Ru; Yang, Feng-Ling; Hsieh, Pei-Fang; Wu, Shih-Hsiung; Wang, Jin-Town.
Afiliación
  • Hsu CR; Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan.
  • Liao CH; Department of Medical Research, E-Da Hospital, Kaohsiung, Taiwan.
  • Lin TL; School of Medicine, I-Shou University, Kaohsiung, Taiwan.
  • Yang HR; Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan.
  • Yang FL; Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan.
  • Hsieh PF; Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan.
  • Wu SH; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan.
  • Wang JT; Department of Microbiology, National Taiwan University College of Medicine, Taipei, Taiwan.
Sci Rep ; 6: 31946, 2016 08 23.
Article en En | MEDLINE | ID: mdl-27550826
Klebsiella pneumoniae can cause community-acquired pyogenic liver abscess (PLA). Capsular polysaccharide (CPS) is important for its virulence. Among 79 capsular (K) types discovered thus far, K57 is often associated with PLA. Here, we report the identification of a K57 variant. Cps gene locus sequencing revealed differences between the K57 reference strain 4425/51 (Ref-K57) and a variant, the PLA isolate A1142. While Ref-K57 cps contained orf13 encoding a putative acetyltransferase, the insertion of a putative transposase-encoding gene at this position was detected in A1142. This variation was detected in other K57 clinical strains. Biochemical analyses indicated that A1142 was deficient in CPS acetylation. Genetic replacement and complementation verified that orf13 was responsible for CPS acetylation. Acetylation increased CPS immunoreactivity to antiserum and enhanced K. pneumoniae induction of pro-inflammatory cytokines through JNK and MAPK signaling. While acetylation diminished the serum resistance of bacteria, it promoted adhesion to intestinal epithelial cells possibly via increasing production of type I fimbriae. In conclusion, acetylation-mediated capsular variation in K57 was observed. Capsular acetylation contributed to the variety and antigenic diversity of CPS, influenced its biological activities, and was involved in K. pneumoniae-host interactions. These findings have implications for vaccine design and pathogenicity of K. pneumoniae.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Klebsiella / Cápsulas Bacterianas / Absceso Piógeno Hepático / Klebsiella pneumoniae Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones por Klebsiella / Cápsulas Bacterianas / Absceso Piógeno Hepático / Klebsiella pneumoniae Tipo de estudio: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido