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The lectin Siglec-G inhibits dendritic cell cross-presentation by impairing MHC class I-peptide complex formation.
Ding, Yuanyuan; Guo, Zhenhong; Liu, Yiqi; Li, Xia; Zhang, Qian; Xu, Xiongfei; Gu, Yan; Zhang, Yi; Zhao, Dezhi; Cao, Xuetao.
Afiliación
  • Ding Y; Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, China.
  • Guo Z; National Key Laboratory of Medical Immunology &Institute of Immunology, Second Military Medical University, Shanghai, China.
  • Liu Y; National Key Laboratory of Medical Molecular Biology &Department of Immunology, Center for Immunotherapy, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
  • Li X; National Key Laboratory of Medical Immunology &Institute of Immunology, Second Military Medical University, Shanghai, China.
  • Zhang Q; Institute of Immunology, Zhejiang University School of Medicine, Hangzhou, China.
  • Xu X; National Key Laboratory of Medical Molecular Biology &Department of Immunology, Center for Immunotherapy, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China.
  • Gu Y; National Key Laboratory of Medical Immunology &Institute of Immunology, Second Military Medical University, Shanghai, China.
  • Zhang Y; National Key Laboratory of Medical Immunology &Institute of Immunology, Second Military Medical University, Shanghai, China.
  • Zhao D; National Key Laboratory of Medical Immunology &Institute of Immunology, Second Military Medical University, Shanghai, China.
  • Cao X; National Key Laboratory of Medical Immunology &Institute of Immunology, Second Military Medical University, Shanghai, China.
Nat Immunol ; 17(10): 1167-75, 2016 10.
Article en En | MEDLINE | ID: mdl-27548433
CD8α(+) dendritic cells (DCs) are specialized at cross-presenting extracellular antigens on major histocompatibility complex (MHC) class I molecules to initiate cytotoxic T lymphocyte (CTL) responses; however, details of the mechanisms that regulate cross-presentation remain unknown. We found lower expression of the lectin family member Siglec-G in CD8α(+) DCs, and Siglec-G deficient (Siglecg(-/-)) mice generated more antigen-specific CTLs to inhibit intracellular bacterial infection and tumor growth. MHC class I-peptide complexes were more abundant on Siglecg(-/-) CD8α(+) DCs than on Siglecg(+/+) CD8α(+) DCs. Mechanistically, phagosome-expressed Siglec-G recruited the phosphatase SHP-1, which dephosphorylated the NADPH oxidase component p47(phox) and inhibited the activation of NOX2 on phagosomes. This resulted in excessive hydrolysis of exogenous antigens, which led to diminished formation of MHC class I-peptide complexes for cross-presentation. Therefore, Siglec-G inhibited DC cross-presentation by impairing such complex formation, and our results add insight into the regulation of cross-presentation in adaptive immunity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Receptores de Antígenos de Linfocitos B / Linfocitos T Citotóxicos / Reactividad Cruzada / Lectinas / Listeriosis / Listeria monocytogenes / Neoplasias Experimentales Límite: Animals Idioma: En Revista: Nat Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Dendríticas / Receptores de Antígenos de Linfocitos B / Linfocitos T Citotóxicos / Reactividad Cruzada / Lectinas / Listeriosis / Listeria monocytogenes / Neoplasias Experimentales Límite: Animals Idioma: En Revista: Nat Immunol Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos