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Sevoflurane represses the self-renewal ability by regulating miR-7a,7b/Klf4 signalling pathway in mouse embryonic stem cells.
Wang, Qimin; Li, Guifeng; Li, Baolin; Chen, Qiu; Lv, Dongdong; Liu, Jiaying; Ma, Jieyu; Sun, Nai; Yang, Longqiu; Fei, Xuejie; Song, Qiong.
Afiliación
  • Wang Q; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Li G; Department of Anesthesiology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, China.
  • Li B; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Chen Q; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Lv D; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Liu J; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Ma J; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Sun N; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Yang L; Department of Anesthesiology, Zhengzhou Central Hospital, Zhengzhou, Henan Province, China.
  • Fei X; Department of Hospital Infections, Shuguang Hospital Affiliated with Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China.
  • Song Q; Department of Hospital Infections, Shuguang Hospital Affiliated with Shanghai University of Traditional Chinese Medicine, Shanghai, 200021, China. doctorsq@126.com.
Cell Prolif ; 49(5): 609-17, 2016 Oct.
Article en En | MEDLINE | ID: mdl-27535693
Sevoflurane is a frequently-used clinical inhalational anaesthetic and can cause toxicity to embryos during foetal development. Embryonic stem cells (ESCs) are derived from the inner cell mass of blastospheres and can be used as a useful model of early development. Here, we found that sevoflurane significantly influenced self-renewal ability of mESCs on stemness maintenance and cell proliferation. The cell cycle was arrested via G1 phase delay. We further found that sevoflurane upregulated expression of miR-7a,7b to repress self-renewal. Next we performed rescue experiments and found that after adding miR-7a,7b inhibitor into mESCs treated with sevoflurane, its influence on self-renewal could be blocked. Further we identified stemness factor Klf4 as the direct target of miR-7a,7b. Overexpression of Klf4 restored self-renewal ability repressed by miR-7a,7b or sevoflurane. In this work, we determined that sevoflurane repressed self-renewal ability by regulating the miR-7a,7b/Klf4 signalling pathway in mESCs. Our study demonstrated molecular mechanism underlying the side effects of sevoflurane during early development, laying the foundation for studies on safe usage of inhalational anaesthetic during non-obstetric surgery.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anestésicos por Inhalación / MicroARNs / Proliferación Celular / Factores de Transcripción de Tipo Kruppel / Células Madre Embrionarias de Ratones / Éteres Metílicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Prolif Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anestésicos por Inhalación / MicroARNs / Proliferación Celular / Factores de Transcripción de Tipo Kruppel / Células Madre Embrionarias de Ratones / Éteres Metílicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Prolif Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido