Your browser doesn't support javascript.
loading
RBM20 Regulates Circular RNA Production From the Titin Gene.
Khan, Mohsin A F; Reckman, Yolan J; Aufiero, Simona; van den Hoogenhof, Maarten M G; van der Made, Ingeborg; Beqqali, Abdelaziz; Koolbergen, Dave R; Rasmussen, Torsten B; van der Velden, Jolanda; Creemers, Esther E; Pinto, Yigal M.
Afiliación
  • Khan MA; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Reckman YJ; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Aufiero S; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • van den Hoogenhof MM; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • van der Made I; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Beqqali A; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Koolbergen DR; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Rasmussen TB; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • van der Velden J; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Creemers EE; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
  • Pinto YM; From the Department of Experimental Cardiology (M.A.F.K., Y.J.R., S.A., M.M.G.v.d.H., I.v.d.M., A.B., E.E.C., Y.M.P.), Department of Clinical Epidemiology, Biostatistics and Bioinformatics (M.A.F.K., S.A.), and Department of Cardiothoracic Surgery (D.R.K.), Academic Medical Center, Amsterdam, The Ne
Circ Res ; 119(9): 996-1003, 2016 Oct 14.
Article en En | MEDLINE | ID: mdl-27531932
RATIONALE: RNA-binding motif protein 20 (RBM20) is essential for normal splicing of many cardiac genes, and loss of RBM20 causes dilated cardiomyopathy. Given its role in splicing, we hypothesized an important role for RBM20 in forming circular RNAs (circRNAs), a novel class of noncoding RNA molecules. OBJECTIVE: To establish the role of RBM20 in the formation of circRNAs in the heart. METHODS AND RESULTS: Here, we performed circRNA profiling on ribosomal depleted RNA from human hearts and identified the expression of thousands of circRNAs, with some of them regulated in disease. Interestingly, we identified 80 circRNAs to be expressed from the titin gene, a gene that is known to undergo highly complex alternative splicing. We show that some of these circRNAs are dynamically regulated in dilated cardiomyopathy but not in hypertrophic cardiomyopathy. We generated RBM20-null mice and show that they completely lack these titin circRNAs. In addition, in a cardiac sample from an RBM20 mutation carrier, titin circRNA production was severely altered. Interestingly, the loss of RBM20 caused only a specific subset of titin circRNAs to be lost. These circRNAs originated from the RBM20-regulated I-band region of the titin transcript. CONCLUSIONS: We show that RBM20 is crucial for the formation of a subset of circRNAs that originate from the I-band of the titin gene. We propose that RBM20, by excluding specific exons from the pre-mRNA, provides the substrate to form this class of RBM20-dependent circRNAs.
Asunto(s)
Palabras clave
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Proteínas de Unión al ARN / Conectina Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Circ Res Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Buscar en Google
Añadir a Mi BVS
Imprimir
XML
PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN / Proteínas de Unión al ARN / Conectina Límite: Adult / Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Circ Res Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos