Creactive protein/oxidized low density lipoprotein/ß2glycoprotein i complexes induce lipid accumulation and inflammatory reaction in macrophages via p38/mitogenactivated protein kinase and nuclear factorκB signaling pathways.
Mol Med Rep
; 14(4): 3490-8, 2016 Oct.
Article
en En
| MEDLINE
| ID: mdl-27512978
Oxidized low-density lipoprotein (oxLDL) can bind to ß2-glycoprotein I (ß2GPI) and C-reactive protein (CRP) to form stable complexes, which exert certain effects in diabetic cardiovascular disease. A previous study by our group has confirmed that the resulting complexes promote atherosclerosis in diabetic BALB/c mice. The present study was designed to investigate the effects and potential mechanisms of oxLDL complexes on lipid accumulation and inflammatory reactions in RAW264.7 macrophages cultured in a hyperglycemic environment. Cultured cells were divided into seven groups, which were treated with phosphatebuffered saline (control), CRP, ß2GPI, oxLDL, CRP/oxLDL, oxLDL/ß2GPI or CRP/oxLDL/ß2GPI. The results revealed the formation of foam cells in the oxLDL, CRP/oxLDL, oxLDL/ß2GPI as well as CRP/oxLDL/ß2GPI groups. Compared with oxLDL, the three complexes induced less lipid accumulation (P<0.05) through inhibiting the expression of CD36 mRNA and promoting the expression of and ABCG1 mRNA (P<0.05 vs. oxLDL). Furthermore, the levels of inflammatory factors interleukin (IL)1ß, IL6 and tumor necrosis factorα were elevated in the CRP/oxLDL and CRP/oxLDL/ß2GPI groups (P>0.05 vs. oxLDL), and obvious effects on p38/mitogenactivated protein kinase and nuclear factor (NF)κB phosphorylation were also observed in these groups (P<0.05 vs. oxLDL). These results suggested that CRP/oxLDL/ßG2P1 complexes may induce lipid accumulation and inflammation in macrophages via the p38/MAPK and NFκB signaling pathways. However, some differences were observed between the complexes, which may be attributed to the property of each constituent; therefore, further studies are required.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteína C-Reactiva
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FN-kappa B
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Beta 2 Glicoproteína I
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Inflamación
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Lípidos
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Lipoproteínas LDL
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Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Grecia