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Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice.
Kalliokoski, Suvi; Piqueras, Victoria Ortín; Frías, Rafael; Sulic, Ana-Marija; Määttä, Juha A E; Kähkönen, Niklas; Viiri, Keijo; Huhtala, Heini; Pasternack, Arja; Laurila, Kaija; Sblattero, Daniele; Korponay-Szabó, Ilma R; Mäki, Markku; Caja, Sergio; Kaukinen, Katri; Lindfors, Katri.
Afiliación
  • Kalliokoski S; Tampere Center for Child Health Research, School of Medicine, University of Tampere and Tampere University Hospital, PL 100, 33014, Tampere, Finland. suvi.kalliokoski@uta.fi.
  • Piqueras VO; Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, 00014, Helsinki, Finland.
  • Frías R; Central Animal Laboratory, University of Turku, 20520, Turku, Finland.
  • Sulic AM; Central Animal Laboratory, University of Turku, 20520, Turku, Finland.
  • Määttä JA; Comparative Medicine, Karolinska Institutet, 17177, Stockholm, Sweden.
  • Kähkönen N; Tampere Center for Child Health Research, School of Medicine, University of Tampere and Tampere University Hospital, PL 100, 33014, Tampere, Finland.
  • Viiri K; BioMediTech, University of Tampere, 33014, Tampere, Finland.
  • Huhtala H; BioMediTech, University of Tampere, 33014, Tampere, Finland.
  • Pasternack A; Tampere Center for Child Health Research, School of Medicine, University of Tampere and Tampere University Hospital, PL 100, 33014, Tampere, Finland.
  • Laurila K; Tampere School of Health Sciences, University of Tampere, 33014, Tampere, Finland.
  • Sblattero D; Department of Bacteriology and Immunology, University of Helsinki, 00014, Helsinki, Finland.
  • Korponay-Szabó IR; Tampere Center for Child Health Research, School of Medicine, University of Tampere and Tampere University Hospital, PL 100, 33014, Tampere, Finland.
  • Mäki M; Department of Life Sciences, University of Trieste, 34100, Trieste, Italy.
  • Caja S; Tampere Center for Child Health Research, School of Medicine, University of Tampere and Tampere University Hospital, PL 100, 33014, Tampere, Finland.
  • Kaukinen K; Coeliac Disease Center, Medical and Health Science Center, Heim Pál Children's Hospital and Department of Pediatrics, University of Debrecen, 4032, Debrecen, Hungary.
  • Lindfors K; Tampere Center for Child Health Research, School of Medicine, University of Tampere and Tampere University Hospital, PL 100, 33014, Tampere, Finland.
Amino Acids ; 49(3): 529-540, 2017 03.
Article en En | MEDLINE | ID: mdl-27503559
Coeliac disease is hallmarked by an abnormal immune reaction against ingested wheat-, rye- and barley-derived gluten and the presence of transglutaminase 2 (TG2)-targeted autoantibodies. The small-bowel mucosal damage characteristic of the disorder develops gradually from normal villus morphology to inflammation and finally to villus atrophy with crypt hyperplasia. Patients with early-stage coeliac disease have TG2-autoantibodies present in serum and small-intestinal mucosa and they may already suffer from abdominal symptoms before the development of villus atrophy. Previously, we have shown that intraperitoneal injections of coeliac patient-derived sera or purified immunoglobulin fraction into mice induce a condition mimicking early-stage coeliac disease. In the current study, we sought to establish whether recombinantly produced patient-derived TG2-targeted autoantibodies are by themselves sufficient for the development of such an experimentally induced condition in immune-compromised mice. Interestingly, mice injected with coeliac patient TG2-antibodies had altered small-intestinal mucosal morphology, increased lamina propria cellular infiltration and disease-specific autoantibodies deposited in the small bowel, but did not evince clinical features of the disease. Thus, coeliac patient-derived TG2-specific autoantibodies seem to be sufficient for the induction of subtle small-bowel mucosal alterations in mice, but the development of clinical features probably requires additional factors such as other antibody populations relevant in coeliac disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autoanticuerpos / Enfermedad Celíaca / Transglutaminasas / Huésped Inmunocomprometido / Proteínas de Unión al GTP / Mucosa Intestinal / Intestino Delgado / Anticuerpos Monoclonales Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Amino Acids Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Austria
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autoanticuerpos / Enfermedad Celíaca / Transglutaminasas / Huésped Inmunocomprometido / Proteínas de Unión al GTP / Mucosa Intestinal / Intestino Delgado / Anticuerpos Monoclonales Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Amino Acids Asunto de la revista: BIOQUIMICA Año: 2017 Tipo del documento: Article País de afiliación: Finlandia Pais de publicación: Austria