Your browser doesn't support javascript.
loading
Repair of UVB-induced DNA damage is reduced in melanoma due to low XPC and global genome repair.
Budden, Timothy; Davey, Ryan J; Vilain, Ricardo E; Ashton, Katie A; Braye, Stephen G; Beveridge, Natalie J; Bowden, Nikola A.
Afiliación
  • Budden T; Hunter Medical Research Institute and Faculty of Health, University of Newcastle, Callaghan, NSW, Australia.
  • Davey RJ; Hunter Medical Research Institute and Faculty of Health, University of Newcastle, Callaghan, NSW, Australia.
  • Vilain RE; Hunter Medical Research Institute and Faculty of Health, University of Newcastle, Callaghan, NSW, Australia.
  • Ashton KA; Melanoma Institute of Australia, Camperdown, NSW, Australia.
  • Braye SG; Sydney Medical School, University of Sydney, Sydney, NSW, Australia.
  • Beveridge NJ; Hunter Area Pathology Service, Pathology North, John Hunter Hospital, Newcastle, NSW, Australia.
  • Bowden NA; Hunter Medical Research Institute and Faculty of Health, University of Newcastle, Callaghan, NSW, Australia.
Oncotarget ; 7(38): 60940-60953, 2016 Sep 20.
Article en En | MEDLINE | ID: mdl-27487145
UVB exposure leads to DNA damage, which when unrepaired induces C>T transitions. These mutations are found throughout the melanoma genome, particularly in non-transcribed regions. The global genome repair (GGR) branch of nucleotide excision repair (NER) is responsible for repairing UV-induced DNA damage across non-transcribed and silent regions of the genome. This study aimed to examine the relationship between UVB and GGR in melanoma. DNA repair capacity and relative expression of NER in melanocytes and melanoma cell lines before and after treatment with UVB was quantified. Transcript expression from 196 melanomas was compared to clinical parameters including solar elastosis and whole transcriptome data collected. Melanoma cell lines showed significantly reduced DNA repair when compared to melanocytes, most significantly in the S phase of the cell cycle. Expression of GGR components XPC, DDB1 and DDB2 was significantly lower in melanoma after UVB. In the melanoma tumours, XPC expression correlated with age of diagnosis and low XPC conferred significantly poorer survival. The same trend was seen in the TCGA melanoma dataset. Reduced GGR in melanoma may contribute to the UV mutation spectrum of the melanoma genome and adds further to the growing evidence of the link between UV, NER and melanoma.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Daño del ADN / Proteínas de Unión al ADN / Reparación del ADN / Melanoma Límite: Humans Idioma: En Revista: Oncotarget Año: 2016 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Cutáneas / Daño del ADN / Proteínas de Unión al ADN / Reparación del ADN / Melanoma Límite: Humans Idioma: En Revista: Oncotarget Año: 2016 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos