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Prostaglandin E2 exerts the proapoptotic and antiproliferative effects on bovine NK cells.
Maslanka, Tomasz; Chrostowska, Malgorzata; Otrocka-Domagala, Iwona; Snarska, Anna; Mikiewicz, Mateusz; Zuska-Prot, Monika; Jasiecka, Agnieszka; Ziólkowski, Hubert; Markiewicz, Wlodzimierz; Jaroszewski, Jerzy J.
Afiliación
  • Maslanka T; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland. Electronic address: tomasz.maslanka@uwm.edu.pl.
  • Chrostowska M; Wipasz S.A., Wadag 9, 10-373 Olsztyn, Poland.
  • Otrocka-Domagala I; Department of Pathological Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
  • Snarska A; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 14, 10-719 Olsztyn, Poland.
  • Mikiewicz M; Department of Internal Medicine, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 14, 10-719 Olsztyn, Poland.
  • Zuska-Prot M; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
  • Jasiecka A; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
  • Ziólkowski H; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
  • Markiewicz W; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
  • Jaroszewski JJ; Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego Street 13, 10-719 Olsztyn, Poland.
Res Vet Sci ; 107: 80-87, 2016 Aug.
Article en En | MEDLINE | ID: mdl-27473979
The aim of this research was to determine whether prostaglandin E2 (PGE2) affects bovine NK cells in respect of their counts, apoptosis and proliferation, and if it does, then which of the PGE2 receptor (EP) subtype(s) mediate(s) these effects. We here report that long-term, but not short-term, exposure of bovine peripheral blood mononuclear cells to PGE2 at 10(-5)M, 10(-6)M and 10(-7)M, but not at 10(-8)M, caused a significant increase in the percentage of early apoptotic cells among NK cell subset. Moreover, PGE2 at 10(-5)M and 10(-6)M, but not at 10(-7)M and 10(-8)M, induced a considerable decrease in the absolute count of NK cells. The magnitude of these effects increased with an increasing concentration of PGE2. The blockade of EP1, EP2, EP3 and EP4 receptors did not prevent the PGE2-induced apoptosis and depletion of NK cells. The results suggest that the proapoptotic effect of PGE2 is secondary in character and the induction of this effect is not mediated through EP receptors. Furthermore, the studies demonstrated that PGE2 at 10(-5)M and 10(-6)M, but not at 10(-7)M and 10(-8)M, highly significantly reduced the percentage of proliferating NK cells. The EP1, EP1/2 and EP3 receptor antagonists were unable to block this effect significantly, whereas the selective blockade of EP4 receptors prevented the PGE2-induced inhibition of NK cells proliferation. These results indicate that PGE2 at certain concentrations may impair the proliferation of NK cells and this effect is mediated via the EP4 receptor.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bovinos / Células Asesinas Naturales / Dinoprostona / Apoptosis / Proliferación Celular Límite: Animals Idioma: En Revista: Res Vet Sci Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bovinos / Células Asesinas Naturales / Dinoprostona / Apoptosis / Proliferación Celular Límite: Animals Idioma: En Revista: Res Vet Sci Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido