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Comparative transcriptomics of rice plants under cold, iron, and salt stresses.
do Amaral, Marcelo Nogueira; Arge, Luis Willian Pacheco; Benitez, Letícia Carvalho; Danielowski, Rodrigo; Silveira, Solange Ferreira da Silveira; Farias, Daniel da Rosa; de Oliveira, Antonio Costa; da Maia, Luciano Carlos; Braga, Eugenia Jacira Bolacel.
Afiliación
  • do Amaral MN; Departament of Botany, Federal University of Pelotas, Pelotas, RS, Brazil. theoamaral@hotmail.com.
  • Arge LW; Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Benitez LC; Departament of Botany, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Danielowski R; Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Silveira SF; Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Farias Dda R; Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil.
  • de Oliveira AC; Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil.
  • da Maia LC; Plant Breeding Center, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Braga EJ; Departament of Botany, Federal University of Pelotas, Pelotas, RS, Brazil.
Funct Integr Genomics ; 16(5): 567-79, 2016 Sep.
Article en En | MEDLINE | ID: mdl-27468828
Abiotic stresses such as salinity, iron toxicity, and low temperatures are the main limiting factors of rice (Oryza sativa L.) yield. The elucidation of the genes involved in responses to these stresses is extremely important to understand the mechanisms that confer tolerance, as well as for the development of cultivars adapted to these conditions. In this study, the RNA-seq technique was used to compare the transcriptional profile of rice leaves (cv. BRS Querência) in stage V3, exposed to cold, iron, and salt stresses for 24 h. A range of 41 to 51 million reads was aligned, in which a total range of 88.47 to 89.21 % was mapped in the reference genome. For cold stress, 7905 differentially expressed genes (DEGs) were observed, 2092 for salt and 681 for iron stress; 370 of these were common to the three DEG stresses. Functional annotation by software MapMan demonstrated that cold stress usually promoted the greatest changes in the overall metabolism, and an enrichment analysis of overrepresented gene ontology (GO) terms showed that most of them are contained in plastids, ribosome, and chloroplasts. Saline stress induced a more complex interaction network of upregulated overrepresented GO terms with a relatively low number of genes compared with cold stress. Our study demonstrated a high number of differentially expressed genes under cold stress and a greater relationship between salt and iron stress levels. The physiological process most affected at the molecular level by the three stresses seems to be photosynthesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Oryza / Estrés Fisiológico / Transcriptoma Idioma: En Revista: Funct Integr Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Oryza / Estrés Fisiológico / Transcriptoma Idioma: En Revista: Funct Integr Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania