HIC1 modulates uveal melanoma progression by activating lncRNA-numb.

Cheng, Guangcun; He, Jie; Zhang, Leilei; Ge, Shengfang; Zhang, He; Fan, Xianqun

Cheng, Guangcun; He, Jie; Zhang, Leilei; Ge, Shengfang; Zhang, He; Fan, Xianqun.

Afiliación

Cheng G; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
He J; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Zhang L; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Ge S; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Zhang H; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. zhanghe@sjtu.edu.cn.
Fan X; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. fanxq@sh163.net.

Tumour Biol ; 37(9): 12779-12789, 2016 Sep.

Article en En | MEDLINE | ID: mdl-27449031

RESUMEN

Uveal melanoma (UM) is the most common primary intraocular cancer in adults. Although the diagnosis modality of primary UM was improved significantly, there are currently no effective therapies for metastatic UM. Hypermethylated in cancer 1 (HIC1) is frequently deleted or epigenetically silenced in various human cancers. However, the role and mechanism of HIC1 in UM is still unclear. In this study, we found that HIC1 acted as a tumor suppressor and that its expression was downregulated in UM. Functional studies demonstrated that ectopic expression of HIC1 in UM cells inhibited cell proliferation and invasion. Moreover, through long non-coding RNA (lncRNA) microarray and real-time PCR, we found that expression of lncRNA-numb was activated by HIC1 in UM. The results provide evidence that lncRNA-numb is a newly proposed tumor suppressor that is involved in HIC1-induced phenotypes. Taken together, our studies of UM reveal a critical role of HIC1 in the regulation of tumorigenesis, at least partly through its downstream target, lncRNA-numb, and provide a potential therapeutic target for UM.

Asunto(s)

Regulación Neoplásica de la Expresión Génica; Factores de Transcripción de Tipo Kruppel/genética; Melanoma/genética; ARN Largo no Codificante/genética; Neoplasias de la Úvea/genética; Western Blotting; Línea Celular Tumoral; Proliferación Celular/genética; Progresión de la Enfermedad; Regulación hacia Abajo; Femenino; Perfilación de la Expresión Génica/métodos; Humanos; Inmunohistoquímica; Factores de Transcripción de Tipo Kruppel/metabolismo; Masculino; Melanoma/metabolismo; Melanoma/patología; Persona de Mediana Edad; Análisis de Secuencia por Matrices de Oligonucleótidos/métodos; Reacción en Cadena de la Polimerasa de Transcriptasa Inversa; Neoplasias de la Úvea/metabolismo; Neoplasias de la Úvea/patología

Palabras clave

HIC1; Long non-coding RNA; Numb; Uveal melanoma

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Úvea / Regulación Neoplásica de la Expresión Génica / Factores de Transcripción de Tipo Kruppel / ARN Largo no Codificante / Melanoma Límite: Female / Humans / Male / Middle aged Idioma: En Revista: Tumour Biol Asunto de la revista: NEOPLASIAS Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

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