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Lactobacillus rhamnosus induced epithelial cell apoptosis, ameliorates inflammation and prevents colon cancer development in an animal model.
Gamallat, Yaser; Meyiah, Abdo; Kuugbee, Eugene D; Hago, Ahmed Musa; Chiwala, Gift; Awadasseid, Annoor; Bamba, Djibril; Zhang, Xin; Shang, Xueqi; Luo, Fuwen; Xin, Yi.
Afiliación
  • Gamallat Y; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Meyiah A; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Kuugbee ED; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Hago AM; Department of Pathology and Pathophysiology, Dalian Medical University, Dalian 116044, China.
  • Chiwala G; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Awadasseid A; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Bamba D; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Zhang X; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Shang X; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China.
  • Luo F; Department of Acute abdominal Surgery, Second Affiliated Hospital of Dalian Medical University, China. Electronic address: fuwenluo@aliyun.com.
  • Xin Y; Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, China. Electronic address: jimxin@hotmail.com.
Biomed Pharmacother ; 83: 536-541, 2016 Oct.
Article en En | MEDLINE | ID: mdl-27447122
BACKGROUND/AIM: Probiotics have been suggested as prophylactic measure in colon carcinogenesis. This study aimed at determining the potential prophylactic activity of Lactobacillus rhamnosus GG CGMCC 1.2134 (LGG) strain on colorectal carcinogenesis via measuring its effect on Nuclear factor kappa B (NFκB) inflammatory pathway and apoptosis. MATERIALS AND METHODS: 64 Sprague Dawley rats were grouped into four as follows; Group 1 (Healthy control), Group 2 (LGG), Group 3 (cancer control Dimethyl hydrazine (DMH)) and Group 4 (LGG+DMH). LGG was administered orally to LGG and LGG+DMH groups. Colon carcinogenesis was chemically induced in LGG+DMH and DMH groups by weekly injection of 40mg/kg DMH. Animals were sacrificed after 25 weeks of experiment and tumor characteristics assessed. The change in expression of NFκB-p65, COX-2, TNFα, Bcl-2, Bax, iNOS, VEGFα, ß-catenin, Casp3 and p53 were evaluated by western blotting and qRT-PCR. RESULTS: LGG treatment significantly reduced tumor incidence, multiplicity and volume in LGG+DMH treatment group compared to DMH cancer control group. Also, LGG treatment reduced the expression of ß-catenin and the inflammatory proteins NFκB-p65, COX-2 and TNFα; the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic proteins Bax, casp3 and p53 compared with DMH group. CONCLUSION: LGG have a potential protection effect against colon carcinogenesis; inducing apoptosis and ameliorating inflammation, and may hold a promise as bio-therapeutic dietary agent.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apoptosis / Neoplasias del Colon / Células Epiteliales / Lacticaseibacillus rhamnosus / Carcinogénesis / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biomed Pharmacother Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Francia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Apoptosis / Neoplasias del Colon / Células Epiteliales / Lacticaseibacillus rhamnosus / Carcinogénesis / Inflamación Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biomed Pharmacother Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Francia