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Examination of CK2α and NF-κB p65 expression in human benign prostatic hyperplasia and prostate cancer tissues.
Qaiser, Fatima; Trembley, Janeen H; Sadiq, Sarah; Muhammad, Iqbal; Younis, Rubina; Hashmi, Shoaib Naiyar; Murtaza, Badar; Rector, Thomas S; Naveed, Abdul Khaliq; Ahmed, Khalil.
Afiliación
  • Qaiser F; Department of Biochemistry and Molecular Biology, Army Medical College, National University of Sciences and Technology, Islamabad, Pakistan.
  • Trembley JH; Department of Veterans Affairs, Cellular and Molecular Biochemistry Research Laboratory (151) Research Service, Minneapolis VA Health Care System, One Veterans Drive, Minneapolis, MN, 55417, USA.
  • Sadiq S; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA.
  • Muhammad I; Masonic Cancer Center, University of Minnesota, Minneapolis, MN, USA.
  • Younis R; Department of Biochemistry and Molecular Biology, Army Medical College, National University of Sciences and Technology, Islamabad, Pakistan.
  • Hashmi SN; Department of Histopathology, Army Medical College, Rawalpindi, Pakistan.
  • Murtaza B; Department of Histopathology, Army Medical College, Rawalpindi, Pakistan.
  • Rector TS; Department of Histopathology, Armed Forces Institute of Pathology, Combined Military Hospital, Rawalpindi, Pakistan.
  • Naveed AK; Armed Forces Institute of Urology, Combined Military Hospital, Rawalpindi, Pakistan.
  • Ahmed K; Department of Veterans Affairs, Cellular and Molecular Biochemistry Research Laboratory (151) Research Service, Minneapolis VA Health Care System, One Veterans Drive, Minneapolis, MN, 55417, USA.
Mol Cell Biochem ; 420(1-2): 43-51, 2016 Sep.
Article en En | MEDLINE | ID: mdl-27435858
Protein kinase CK2 plays a critical role in cell growth, proliferation, and suppression of cell death. CK2 is overexpressed, especially in the nuclear compartment, in the majority of cancers, including prostate cancer (PCa). CK2-mediated activation of transcription factor nuclear factor kappa B (NF-κB) p65 is a key step in cellular proliferation, resulting in translocation of NF-κB p65 from the cytoplasm to the nucleus. As CK2 expression and activity are also elevated in benign prostatic hyperplasia (BPH), we sought to increase the knowledge of CK2 function in benign and malignant prostate by examination of the relationships between nuclear CK2 and nuclear NF-κB p65 protein expression. The expression level and localization of CK2α and NF-κB p65 proteins in PCa and BPH tissue specimens was determined. Nuclear CK2α and NF-κB p65 protein levels are significantly higher in PCa compared with BPH, and these proteins are positively correlated with each other in both diseases. Nuclear NF-κB p65 levels correlated with Ki-67 or with cytoplasmic NF-κB p65 expression in BPH, but not in PCa. The findings provide information that combined analysis of CK2α and NF-κB p65 expression in prostate specimens relates to the disease status. Increased nuclear NF-κB p65 expression levels in PCa specifically related to nuclear CK2α levels, indicating a possible CK2-dependent relationship in malignancy. In contrast, nuclear NF-κB p65 protein levels related to both Ki-67 and cytoplasmic NF-κB p65 levels exclusively in BPH, suggesting a potential separate impact for NF-κB p65 function in proliferation for benign disease as opposed to malignant disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hiperplasia Prostática / Neoplasias de la Próstata / Regulación Neoplásica de la Expresión Génica / Núcleo Celular / Quinasa de la Caseína II / Factor de Transcripción ReIA / Proteínas de Neoplasias Límite: Humans / Male Idioma: En Revista: Mol Cell Biochem Año: 2016 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hiperplasia Prostática / Neoplasias de la Próstata / Regulación Neoplásica de la Expresión Génica / Núcleo Celular / Quinasa de la Caseína II / Factor de Transcripción ReIA / Proteínas de Neoplasias Límite: Humans / Male Idioma: En Revista: Mol Cell Biochem Año: 2016 Tipo del documento: Article País de afiliación: Pakistán Pais de publicación: Países Bajos