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Evolutionary dynamics of Anolis sex chromosomes revealed by sequencing of flow sorting-derived microchromosome-specific DNA.
Kichigin, Ilya G; Giovannotti, Massimo; Makunin, Alex I; Ng, Bee L; Kabilov, Marsel R; Tupikin, Alexey E; Barucchi, Vincenzo Caputo; Splendiani, Andrea; Ruggeri, Paolo; Rens, Willem; O'Brien, Patricia C M; Ferguson-Smith, Malcolm A; Graphodatsky, Alexander S; Trifonov, Vladimir A.
Afiliación
  • Kichigin IG; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, 630090, Russia. kig@mcb.nsc.ru.
  • Giovannotti M; Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
  • Makunin AI; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, 630090, Russia.
  • Ng BL; Cytometry Core Facility, The Wellcome Trust Sanger Institute, Genome Campus, Cambridge, UK.
  • Kabilov MR; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, 630090, Russia.
  • Tupikin AE; Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, 630090, Russia.
  • Barucchi VC; Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
  • Splendiani A; Consiglio Nazionale delle Ricerche, Istituto di Scienze Marine Sezione Pesca Marittima, Largo Fiera della Pesca, 60125, Ancona, Italy.
  • Ruggeri P; Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
  • Rens W; Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, via Brecce Bianche, 60131, Ancona, Italy.
  • O'Brien PC; Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
  • Ferguson-Smith MA; Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
  • Graphodatsky AS; Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, CB3 0ES, UK.
  • Trifonov VA; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, 630090, Russia.
Mol Genet Genomics ; 291(5): 1955-66, 2016 Oct.
Article en En | MEDLINE | ID: mdl-27431992
Squamate reptiles show a striking diversity in modes of sex determination, including both genetic (XY or ZW) and temperature-dependent sex determination systems. The genomes of only a handful of species have been sequenced, analyzed and assembled including the genome of Anolis carolinensis. Despite a high genome coverage, only macrochromosomes of A. carolinensis were assembled whereas the content of most microchromosomes remained unclear. Most of the Anolis species have homomorphic XY sex chromosome system. However, some species have large heteromorphic XY chromosomes (e.g., A. sagrei) and even multiple sex chromosomes systems (e.g. A. pogus), that were shown to be derived from fusions of the ancestral XY with microautosomes. We applied next generation sequencing of flow sorting-derived chromosome-specific DNA pools to characterize the content and composition of microchromosomes in A. carolinensis and A. sagrei. Comparative analysis of sequenced chromosome-specific DNA pools revealed that the A. sagrei XY sex chromosomes contain regions homologous to several microautosomes of A. carolinensis. We suggest that the sex chromosomes of A. sagrei are derived by fusions of the ancestral sex chromosome with three microautosomes and subsequent loss of some genetic content on the Y chromosome.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reptiles / Cromosomas Sexuales / Análisis de Secuencia de ADN / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Animals Idioma: En Revista: Mol Genet Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reptiles / Cromosomas Sexuales / Análisis de Secuencia de ADN / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Animals Idioma: En Revista: Mol Genet Genomics Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Alemania