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Anterograde-propagation of axonal degeneration in the visual system of wlds mice characterized by diffusion tensor imaging.
Sun, Shu-Wei; Nishioka, Christopher; Chung, Chen-Fang; Park, JoAnn; Liang, Hsiao-Fang.
Afiliación
  • Sun SW; Basic Sciences, School of Medicine, Loma Linda University, California, USA.
  • Nishioka C; Radiation Medicine, School of Medicine, Loma Linda University, California, USA.
  • Chung CF; Pharmaceutical Science, School of Pharmacy, Loma Linda University, California, USA.
  • Park J; Neuroscience, University of California, Riverside, California, USA.
  • Liang HF; Neuroscience, University of California, Riverside, California, USA.
J Magn Reson Imaging ; 45(2): 482-491, 2017 02.
Article en En | MEDLINE | ID: mdl-27373882
PURPOSE: To evaluate the feasibility of using diffusion tensor imaging (DTI) to characterize the temporospatial profile of axonal degeneration and its relation to blood-brain barrier (BBB) permeability. MATERIALS AND METHODS: Longitudinal DTI was performed in Wallerian degeneration slow (WldS) mice following retinal ischemia. In parallel, gadolinium (Gd)-enhanced T1 -weighted imaging (Gd-T1 WI) was performed to evaluate BBB permeability in white matter during axonal degeneration. To confirm the in vivo findings, immunohistochemistry using SMI-31 and myelin basic protein (MBP) was performed to examine the axons and myelin, respectively, and Evans blue was used to evaluate the permeability of the BBB. RESULTS: Reduced axial diffusivity was found in the optic nerve (ON, -15%, P = 0.0063) 1 week and optic tact (OT, -18%, P = 0.0077) 2 weeks after retinal ischemia, which were respectively associated with an 11% (P = 0.0116) and 25% (P = 0.0001) axonal loss. Increased radial diffusivity was found 1-2 weeks after the colocated decrease of axial diffusivity (35% increase, P = 0.0388 in the ON at week 2 and an 80% increase, P = 0.0015 in the OT at week 4). No significant changes were observed using Gd-T1 WI (P = 0.13-0.75), although an approximately 1-fold increase in Evans blue staining intensity was found in the injured ON and OT starting 1 week after retinal ischemia. CONCLUSION: We demonstrated the utility of DTI to characterize anterograde-propagating axonal degeneration through the ON and OT following retinal damage. Evans blue staining revealed serum albumin accumulation at injured sites, although there was no BBB leakage detectable using Gd-T1 WI. LEVEL OF EVIDENCE: 2 J. Magn. Reson. Imaging 2017;45:482-491.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Degeneración Retiniana / Axones / Vías Visuales / Barrera Hematoencefálica / Lesión Axonal Difusa / Imagen de Difusión Tensora Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Magn Reson Imaging Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Degeneración Retiniana / Axones / Vías Visuales / Barrera Hematoencefálica / Lesión Axonal Difusa / Imagen de Difusión Tensora Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: J Magn Reson Imaging Asunto de la revista: DIAGNOSTICO POR IMAGEM Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos