Three-dimensional architectures of P2X2-/P2X3-immunoreactive afferent nerve terminals in the rat carotid body as revealed by confocal laser scanning microscopy.

Yokoyama, Takuya; Saino, Tomoyuki; Nakamuta, Nobuaki; Kusakabe, Tatsumi; Yamamoto, Yoshio

Yokoyama, Takuya; Saino, Tomoyuki; Nakamuta, Nobuaki; Kusakabe, Tatsumi; Yamamoto, Yoshio.

Afiliación

Yokoyama T; Department of Anatomy (Cell Biology), Iwate Medical University, Yahaba, Japan.
Saino T; Department of Anatomy (Cell Biology), Iwate Medical University, Yahaba, Japan.
Nakamuta N; Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate University, 18-8 Ueda 3-chome, Morioka, Iwate, 020-8550, Japan.
Kusakabe T; Laboratory for Anatomy and Physiology, Department of Sport and Medical Science, Kokushikan University, Tama, Japan.
Yamamoto Y; Laboratory of Veterinary Anatomy and Cell Biology, Faculty of Agriculture, Iwate University, 18-8 Ueda 3-chome, Morioka, Iwate, 020-8550, Japan. yyoshio@iwate-u.ac.jp.

Histochem Cell Biol ; 146(4): 479-88, 2016 Oct.

Article en En | MEDLINE | ID: mdl-27368183

RESUMEN

We investigated the three-dimensional architectures of P2X2-/P2X3-immunoreactive nerve terminals in the rat carotid body using immunohistochemistry with confocal laser microscopy. Nerve endings immunoreactive for P2X2 and P2X3 were associated with clusters of type I cells, whereas some nerve endings were sparsely distributed in a few clusters. Most nerve endings surrounding type I cells were hederiform in shape and extended several flattened axon terminals, which were polygonal or pleomorphic in shape and contained P2X2-/P2X3-immunoreactive products. Three-dimensional reconstruction views revealed that some flattened nerve endings with P2X3 immunoreactivity formed arborized, sac- or goblet-like terminal structures and were attached to type I cells immunoreactive for tyrosine hydroxylase (TH). However, P2X3-immunoreactive axon terminals were sparsely distributed in type I cells immunoreactive for dopamine beta-hydroxylase. Multi-immunolabeling for P2X2, S100, and TH revealed that P2X2-immunoreactive axon terminals were attached to TH-immunoreactive type I cells on the inside of type II cells with S100 immunoreactivity. These results revealed the detailed morphology of P2X2-/P2X3-immunoreactive nerve terminals and suggest that sensory nerve endings may integrate chemosensory signals from clustered type I cells with their variform nerve terminals.

Asunto(s)

Cuerpo Carotídeo/anatomía & histología; Cuerpo Carotídeo/metabolismo; Microscopía Confocal; Terminaciones Nerviosas/metabolismo; Receptores Purinérgicos P2X2/inmunología; Receptores Purinérgicos P2X3/inmunología; Animales; Cuerpo Carotídeo/inmunología; Inmunohistoquímica; Masculino; Terminaciones Nerviosas/inmunología; Ratas; Ratas Wistar; Receptores Purinérgicos P2X2/análisis; Receptores Purinérgicos P2X3/análisis

Palabras clave

Afferent nerve terminal; Carotid body; Immunohistochemistry; P2X purinoreceptor; Type I cells

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cuerpo Carotídeo / Microscopía Confocal / Receptores Purinérgicos P2X2 / Receptores Purinérgicos P2X3 / Terminaciones Nerviosas Límite: Animals Idioma: En Revista: Histochem Cell Biol Asunto de la revista: CITOLOGIA / HISTOCITOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania

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