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Visualization and analysis of gene expression in tissue sections by spatial transcriptomics.
Ståhl, Patrik L; Salmén, Fredrik; Vickovic, Sanja; Lundmark, Anna; Navarro, José Fernández; Magnusson, Jens; Giacomello, Stefania; Asp, Michaela; Westholm, Jakub O; Huss, Mikael; Mollbrink, Annelie; Linnarsson, Sten; Codeluppi, Simone; Borg, Åke; Pontén, Fredrik; Costea, Paul Igor; Sahlén, Pelin; Mulder, Jan; Bergmann, Olaf; Lundeberg, Joakim; Frisén, Jonas.
Afiliación
  • Ståhl PL; Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden. Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Salmén F; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Vickovic S; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Lundmark A; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden. Department of Dental Medicine, Division of Periodontology, Karolinska Institute, SE-141 04 Huddinge, Sweden.
  • Navarro JF; Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden. Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Magnusson J; Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden.
  • Giacomello S; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Asp M; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Westholm JO; Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Box 1031, SE-171 21 Solna, Sweden.
  • Huss M; Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Box 1031, SE-171 21 Solna, Sweden.
  • Mollbrink A; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Linnarsson S; Division of Molecular Neuroscience, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-17177 Stockholm, Sweden.
  • Codeluppi S; Division of Molecular Neuroscience, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-17177 Stockholm, Sweden. Department of Physiology and Pharmacology, Karolinska Institute, SE-17177 Stockholm, Sweden.
  • Borg Å; Division of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, SE-223 81 Lund, Sweden.
  • Pontén F; Department of Immunology, Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden.
  • Costea PI; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Sahlén P; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
  • Mulder J; Science for Life Laboratory, Department of Neuroscience, Karolinska Institute, SE-171 77 Stockholm, Sweden.
  • Bergmann O; Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden.
  • Lundeberg J; Science for Life Laboratory, Division of Gene Technology, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden. joakim.lundeberg@scilifelab.se.
  • Frisén J; Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden.
Science ; 353(6294): 78-82, 2016 Jul 01.
Article en En | MEDLINE | ID: mdl-27365449
Analysis of the pattern of proteins or messengerRNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Análisis de Secuencia de ARN / Perfilación de la Expresión Génica / Transcriptoma Límite: Animals / Female / Humans Idioma: En Revista: Science Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Análisis de Secuencia de ARN / Perfilación de la Expresión Génica / Transcriptoma Límite: Animals / Female / Humans Idioma: En Revista: Science Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos