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Effect of aqueous and particulate silk fibroin in a rat model of experimental colitis.
Rodriguez-Nogales, A; Lozano-Pérez, A A; Aznar-Cervantes, S D; Algieri, F; Garrido-Mesa, J; Garrido-Mesa, N; Vezza, T; Utrilla, M P; Cenis, J L; Rodríguez-Cabezas, M E; Gálvez, J.
Afiliación
  • Rodriguez-Nogales A; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Lozano-Pérez AA; Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca, Murcia, Spain.
  • Aznar-Cervantes SD; Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca, Murcia, Spain.
  • Algieri F; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Garrido-Mesa J; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Garrido-Mesa N; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Vezza T; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Utrilla MP; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Cenis JL; Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), c/Mayor, 1, 30150 La Alberca, Murcia, Spain.
  • Rodríguez-Cabezas ME; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain.
  • Gálvez J; CIBER-EHD, Department of Pharmacology, ibs.GRANADA, Center for Biomedical Research (CIBM), University of Granada, Avenida del Conocimiento s/n, 18100 Armilla Granada, Spain. Electronic address: jgalvez@ugr.es.
Int J Pharm ; 511(1): 1-9, 2016 Sep 10.
Article en En | MEDLINE | ID: mdl-27363935
Silk fibroin (SF) has anti-inflammatory properties and promotes wound healing. Moreover, SF particles act as carriers of active drugs against intestinal inflammation due to their capacity to deliver the compound to the damaged colonic tissue. The present work assesses the effect of SF in the trinitrobenzenesulfonic acid model of rat colitis that resembles human intestinal inflammation. SF (8mg/kg) was administered in aqueous solution orally and in two particulate formats by intrarectal route, following two technologies: spray drying to make microparticles and desolvation in organic solvent to produce nanoparticles. SF treatments ameliorated the colonic damage, reduced neutrophil infiltration and improved the compromised oxidative status of the colon. They also reduced the gene expression of pro-inflammatory cytokines like IL-1ß and the anti-inflammatory cytokine IL-10. Moreover, they improved the intestinal wall integrity by increasing the gene expression of some of its markers (villin, trefoil factor-3 and mucins), thus accelerating the healing. The immunomodulatory properties of SF particles were also tested in vitro in macrophages: they activated the immune response in basal conditions without increasing it after a pro-inflammatory insult. In conclusion, SF particles could be useful as carriers to deliver active drugs to the damaged intestinal colon with additional anti-inflammatory and healing properties.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua / Colitis / Seda / Modelos Animales de Enfermedad / Fibroínas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Pharm Año: 2016 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua / Colitis / Seda / Modelos Animales de Enfermedad / Fibroínas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Pharm Año: 2016 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos