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Increased bone formation in a rabbit long-bone defect model after single local and single systemic application of erythropoietin.
Omlor, Georg W; Kleinschmidt, Kerstin; Gantz, Simone; Speicher, Anja; Guehring, Thorsten; Richter, Wiltrud.
Afiliación
  • Omlor GW; a Center for Orthopedics, Trauma Surgery and Spinal Cord Injury , Heidelberg University Hospital ;
  • Kleinschmidt K; b Research Center for Experimental Orthopedics , Heidelberg University Hospital ;
  • Gantz S; a Center for Orthopedics, Trauma Surgery and Spinal Cord Injury , Heidelberg University Hospital ;
  • Speicher A; b Research Center for Experimental Orthopedics , Heidelberg University Hospital ;
  • Guehring T; c BG Trauma Hospital Ludwigshafen, University of Heidelberg , Germany.
  • Richter W; b Research Center for Experimental Orthopedics , Heidelberg University Hospital ;
Acta Orthop ; 87(4): 425-31, 2016 Aug.
Article en En | MEDLINE | ID: mdl-27348783
Background and purpose - Delayed bone healing with non-union is a common problem. Further options to increase bone healing together with surgery are needed. We therefore evaluated a 1-dose single application of erythropoietin (EPO), applied either locally to the defect or systemically during surgery, in a critical-size rabbit long-bone defect. Material and methods - 19 New Zealand White rabbits received a 15-mm defect in the radius diaphysis. An absorbable gelatin sponge was soaked with saline (control group and systemic treatment group) or EPO (local treatment group) and implanted into the gap. The systemic treatment group received EPO subcutaneously. In vivo micro-CT analysis was performed 4, 8, and 12 weeks postoperatively. Vascularization was evaluated histologically. Results - Semiquantitative histomorphometric and radiological evaluation showed increased bone formation (2.3- to 2.5-fold) in both treatment groups after 12 weeks compared to the controls. Quantitative determination of bone volume and tissue volume showed superior bone healing after EPO treatment at all follow-up time points, with the highest values after 12 weeks in locally treated animals (3.0- to 3.4-fold). More vascularization was found in both EPO treatment groups. Interpretation - Initial single dosing with EPO was sufficient to increase bone healing substantially after 12 weeks of follow-up. Local application inside the defect was most effective, and it can be administered directly during surgery. Apart from effects on ossification, systemic and local EPO treatment leads to increased callus vascularization.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Fracturas del Radio / Regeneración Ósea / Eritropoyetina / Curación de Fractura Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Acta Orthop Asunto de la revista: ORTOPEDIA Año: 2016 Tipo del documento: Article Pais de publicación: Suecia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Fracturas del Radio / Regeneración Ósea / Eritropoyetina / Curación de Fractura Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: Acta Orthop Asunto de la revista: ORTOPEDIA Año: 2016 Tipo del documento: Article Pais de publicación: Suecia