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SUMO proteases ULP1c and ULP1d are required for development and osmotic stress responses in Arabidopsis thaliana.
Castro, Pedro Humberto; Couto, Daniel; Freitas, Sara; Verde, Nuno; Macho, Alberto P; Huguet, Stéphanie; Botella, Miguel Angel; Ruiz-Albert, Javier; Tavares, Rui Manuel; Bejarano, Eduardo Rodríguez; Azevedo, Herlânder.
Afiliación
  • Castro PH; Biosystems and Integrative Sciences Institute (BioISI), Plant Functional Biology Center, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
  • Couto D; Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Departamento Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, 29071, Malaga, Spain.
  • Freitas S; Section for Plant and Soil Science, Department of Plant and Environmental Sciences, University of Copenhagen, 1871, Frederiksberg C, Denmark.
  • Verde N; Biosystems and Integrative Sciences Institute (BioISI), Plant Functional Biology Center, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
  • Macho AP; The Sainsbury Laboratory, Colney Lane, Norwich, NR4 7UH, UK.
  • Huguet S; Biosystems and Integrative Sciences Institute (BioISI), Plant Functional Biology Center, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
  • Botella MA; Biosystems and Integrative Sciences Institute (BioISI), Plant Functional Biology Center, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
  • Ruiz-Albert J; Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Departamento Biología Celular, Genética y Fisiología, Universidad de Málaga, Campus Teatinos, 29071, Malaga, Spain.
  • Tavares RM; Shanghai Center for Plant Stress Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 201602, Shanghai, China.
  • Bejarano ER; Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165, Université d'Evry Val d'Essonne, ERL CNRS 8196, 2 rue G. Crémieux, CP 5708, 91057, Evry Cedex, France.
  • Azevedo H; Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora", Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Departamento Biología Molecular y Bioquímica, Universidad de Málaga, Campus Teatinos, 29071, Malaga, Spain.
Plant Mol Biol ; 92(1-2): 143-59, 2016 Sep.
Article en En | MEDLINE | ID: mdl-27325215
Sumoylation is an essential post-translational regulator of plant development and the response to environmental stimuli. SUMO conjugation occurs via an E1-E2-E3 cascade, and can be removed by SUMO proteases (ULPs). ULPs are numerous and likely to function as sources of specificity within the pathway, yet most ULPs remain functionally unresolved. In this report we used loss-of-function reverse genetics and transcriptomics to functionally characterize Arabidopsis thaliana ULP1c and ULP1d SUMO proteases. GUS reporter assays implicated ULP1c/d in various developmental stages, and subsequent defects in growth and germination were uncovered using loss-of-function mutants. Microarray analysis evidenced not only a deregulation of genes involved in development, but also in genes controlled by various drought-associated transcriptional regulators. We demonstrated that ulp1c ulp1d displayed diminished in vitro root growth under low water potential and higher stomatal aperture, yet leaf transpirational water loss and whole drought tolerance were not significantly altered. Generation of a triple siz1 ulp1c ulp1d mutant suggests that ULP1c/d and the SUMO E3 ligase SIZ1 may display separate functions in development yet operate epistatically in response to water deficit. We provide experimental evidence that Arabidopsis ULP1c and ULP1d proteases act redundantly as positive regulators of growth, and operate mainly as isopeptidases downstream of SIZ1 in the control of water deficit responses.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis / Osmorregulación Idioma: En Revista: Plant Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2016 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis / Osmorregulación Idioma: En Revista: Plant Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2016 Tipo del documento: Article País de afiliación: Portugal Pais de publicación: Países Bajos