[An Efficient Method for Genetic Certification of Bacillus subtilis strains, Prospective Producers of Biopreparations].
Mikrobiologiia
; 85(1): 50-5, 2016.
Article
en Ru
| MEDLINE
| ID: mdl-27301128
Genetic certification of commercial strains of bacteria antagonistic to phytopathogenic microorganisms guarantees their unequivocal identification and confirmation of safety. In Russia, unlike EU countries, genetic certification of Bacillus subtilis strains is not used. Based on the previously proposed double digestion selective label (DDSL) fingerprinting, a method for genetic identification and certification of B. subtilis strains was proposed. The method was tested on several strains differing in their physiological and biochemical properties and in the composition of secondary metabolites responsible for the spectrum of antibiotic activity. High resolving power of this approach was shown. Optimal restriction endonucleases (SgsI and Eco32I) were determined and validated. A detailed protocol for genetic certification of this bacterial species was developed. DDSL is a universal method, which may be adapted for genetic identification and certification of other bacterial species.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
/
Dermatoglifia del ADN
Idioma:
Ru
Revista:
Mikrobiologiia
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Rusia