Bioluminescent flow sensors: L-lactate dehydrogenase activity determination in serum.

Girotti, S; Bassoli, C; Cascione, M L; Ghini, S; Carrea, G; Bovara, R; Roda, A; Motta, R; Petilino, R

Girotti, S; Bassoli, C; Cascione, M L; Ghini, S; Carrea, G; Bovara, R; Roda, A; Motta, R; Petilino, R.

Afiliación

Girotti S; Institute of Chemical Sciences, University of Bologna, Italy.

J Biolumin Chemilumin ; 3(2): 41-5, 1989.

Article en En | MEDLINE | ID: mdl-2728910

RESUMEN

The catalytic activity of serum L-lactate dehydrogenase (LDH), was determined by monitoring the NADH produced by LDH with bacterial bioluminescent enzymes immobilized on a nylon coil. The LDH reaction of L-lactate with NAD took place in a flow-through mixing coil that preceded the bioluminescent detector coil. The response was linear from 1 to 5000 U/l at 37 degrees C and from 3 to 2000 U/l at 25 degrees C. The intra- and inter-assay reproducibility (CV%) were less than 10% and recovery range was 92% to 110%. The results agreed well with those obtained with a spectrophotometric method.

Asunto(s)

Técnicas Biosensibles; L-Lactato Deshidrogenasa/sangre; Mediciones Luminiscentes; Enzimas Inmovilizadas; Humanos; NAD/metabolismo; Oxidación-Reducción

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / L-Lactato Deshidrogenasa / Mediciones Luminiscentes Límite: Humans Idioma: En Revista: J Biolumin Chemilumin Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 1989 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

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