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Characterization and analysis of a de novo transcriptome from the pygmy grasshopper Tetrix japonica.
Qiu, Zhongying; Liu, Fei; Lu, Huimeng; Huang, Yuan.
Afiliación
  • Qiu Z; College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.
  • Liu F; College of Life Sciences, Shaanxi Normal University, Xi'an, 710062, China.
  • Lu H; College of Life Sciences and Food Engineering, Shaanxi Xueqian Normal University, Xi'an, 710061, China.
  • Huang Y; Key Laboratory for Space Bioscience & Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, China.
Mol Ecol Resour ; 17(3): 381-392, 2017 May.
Article en En | MEDLINE | ID: mdl-27288670
The pygmy grasshopper Tetrix japonica is a common insect distributed throughout the world, and it has the potential for use in studies of body colour polymorphism, genomics and the biology of Tetrigoidea (Insecta: Orthoptera). However, limited biological information is available for this insect. Here, we conducted a de novo transcriptome study of adult and larval T. japonica to provide a better understanding of its gene expression and develop genomic resources for future work. We sequenced and explored the characteristics of the de novo transcriptome of T. japonica using Illumina HiSeq 2000 platform. A total of 107 608 206 paired-end clean reads were assembled into 61 141 unigenes using the trinity software; the mean unigene size was 771 bp, and the N50 length was 1238 bp. A total of 29 225 unigenes were functionally annotated to the NCBI nonredundant protein sequences (Nr), NCBI nonredundant nucleotide sequences (Nt), a manually annotated and reviewed protein sequence database (Swiss-Prot), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. A large number of putative genes that are potentially involved in pigment pathways, juvenile hormone (JH) metabolism and signalling pathways were identified in the T. japonica transcriptome. Additionally, 165 769 and 156 796 putative single nucleotide polymorphisms occurred in the adult and larvae transcriptomes, respectively, and a total of 3162 simple sequence repeats were detected in this assembly. This comprehensive transcriptomic data for T. japonica will provide a usable resource for gene predictions, signalling pathway investigations and molecular marker development for this species and other pygmy grasshoppers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcriptoma / Saltamontes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Ecol Resour Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transcriptoma / Saltamontes Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Mol Ecol Resour Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido