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Assessment of the vaginal residence time of biomarkers of semen exposure.
Thurman, Andrea; Jacot, Terry; Melendez, Johan; Kimble, Thomas; Snead, Margaret; Jamshidi, Roxanne; Wheeless, Angie; Archer, David F; Doncel, Gustavo F; Mauck, Christine.
Afiliación
  • Thurman A; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA. Electronic address: thurmaar@evms.edu.
  • Jacot T; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Melendez J; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Kimble T; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Snead M; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Jamshidi R; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Wheeless A; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Archer DF; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Doncel GF; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
  • Mauck C; Eastern Virginia Medical School, 601 Colley Avenue, Room 235, Norfolk, VA 23507, USA.
Contraception ; 94(5): 512-520, 2016 11.
Article en En | MEDLINE | ID: mdl-27259675
OBJECTIVE: The primary objective of this pilot study is to determine and compare the residence time in the vagina of biomarkers of semen exposure for up to 15 days post exposure. The biomarkers are prostate-specific antigen (PSA), Y chromosome DNA, the sex determining region of the Y chromosome (SRY) and testis-specific protein Y-encoded 4 (TSPY4). The secondary objectives are to determine if biomarker concentrations differed between intercourse and inoculation groups, to establish whether the sampling frequency post exposure affected biomarker concentrations and decay profile and to determine if biomarker concentrations in vaginal swabs obtained by the participant at home were similar to swabs obtained by the nurse in the clinic. STUDY DESIGN: We randomized healthy women to unprotected intercourse (n=17) versus vaginal inoculation with the male partner's semen in the clinic (n=16). Women were then further randomized to have vaginal swabs obtained at either 7 or 4 time points after semen exposure, up to 15 days post exposure, either obtained at home by the participant or in the clinic by the research nurse. RESULTS: PSA and SRY were markers of recent semen exposure. TSPY4 was detectable in approximately 50% of participants at 15 days post exposure. Unprotected intercourse resulted in significantly higher concentrations of select biomarkers. Sampling frequency and home versus clinic sampling had no significant effect on biomarker concentrations. CONCLUSIONS: Objective biomarkers of recent or distant semen exposure may have great utility for verifying protocol compliance in a variety of clinical trials.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Vagina / Antígeno Prostático Específico / Proteínas de Ciclo Celular / Proteína de la Región Y Determinante del Sexo Tipo de estudio: Clinical_trials / Guideline Límite: Adult / Female / Humans / Male Idioma: En Revista: Contraception Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Vagina / Antígeno Prostático Específico / Proteínas de Ciclo Celular / Proteína de la Región Y Determinante del Sexo Tipo de estudio: Clinical_trials / Guideline Límite: Adult / Female / Humans / Male Idioma: En Revista: Contraception Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos