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H2O2 generated from mitochondrial electron transport chain in thoracic perivascular adipose tissue is crucial for modulation of vascular smooth muscle contraction.
Costa, Rafael M; Filgueira, Fernando P; Tostes, Rita C; Carvalho, Maria Helena C; Akamine, Eliana H; Lobato, Nubia S.
Afiliación
  • Costa RM; Department of Pharmacology, Medical School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil; Department of Medicine, Federal University of Goias, Jatai, GO, Brazil; Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil. Ele
  • Filgueira FP; Department of Medicine, Federal University of Goias, Jatai, GO, Brazil.
  • Tostes RC; Department of Pharmacology, Medical School of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, Brazil.
  • Carvalho MH; Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
  • Akamine EH; Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, SP, Brazil.
  • Lobato NS; Department of Medicine, Federal University of Goias, Jatai, GO, Brazil.
Vascul Pharmacol ; 84: 28-37, 2016 09.
Article en En | MEDLINE | ID: mdl-27252154
The perivascular adipose tissue (PVAT) releases a variety of factors that affect vascular function. PVAT in the thoracic aorta shares characteristics with the brown adipose tissue, including a large amount of mitochondria. PVAT-derived factors influence both endothelial and smooth muscle function via several signaling mechanisms including the release/generation of reactive nitrogen and oxygen species. Considering the importance of reactive oxygen species (ROS) on vascular function and that mitochondria are an important source of ROS, we hypothesized that mitochondria-derived ROS in the PVAT modulates vascular reactivity. Vascular reactivity to norephinephrine (NE) was evaluated in thoracic aortic rings, with or without endothelium and/or PVAT, from male Wistar rats. Mitochondrial uncoupling, as well as hydrogen peroxide (H2O2) removal, increased the contraction in vessels surrounded by PVAT. PVAT stimulated with NE exhibited increased protein expression, determined by Western blot analysis, of manganese superoxide dismutase (Mn-SOD) and decreased protein expression of catalase. Ultimately, NE increased superoxide anion (O2(-)) generation in PVAT via increases in intracellular calcium. These results clearly demonstrate that mitochondrial electron transport chain (mETC) in PVAT contributes to modulation of aortic muscle contraction by generating higher amounts of O2(-) that is, in turn, dismutated to hydrogen peroxide, which then acts as a pivotal signaling molecule regulating vascular smooth muscle contraction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aorta Torácica / Tejido Adiposo / Transporte de Electrón / Músculo Liso Vascular Límite: Animals Idioma: En Revista: Vascul Pharmacol Asunto de la revista: ANGIOLOGIA / FARMACOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Aorta Torácica / Tejido Adiposo / Transporte de Electrón / Músculo Liso Vascular Límite: Animals Idioma: En Revista: Vascul Pharmacol Asunto de la revista: ANGIOLOGIA / FARMACOLOGIA Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos