Hypomethylation of let-7a-3 is associated with poor prognosis in myelodysplastic syndrome.

Wu, De-Hong; Yao, Dong-Ming; Yang, Lei; Ma, Ji-Chun; Wen, Xiang-Mei; Yang, Jing; Guo, Hong; Li, Xi-Xi; Qian, Wei; Lin, Jiang; Qian, Jun

Wu, De-Hong; Yao, Dong-Ming; Yang, Lei; Ma, Ji-Chun; Wen, Xiang-Mei; Yang, Jing; Guo, Hong; Li, Xi-Xi; Qian, Wei; Lin, Jiang; Qian, Jun.

Afiliación

Wu DH; a Department of Hematology , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Yao DM; c Department of Hematology , the Third People's Hospital of Kunshan City , Kunshan , China.
Yang L; b Laboratory Center , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Ma JC; a Department of Hematology , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Wen XM; b Laboratory Center , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Yang J; b Laboratory Center , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Guo H; a Department of Hematology , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Li XX; b Laboratory Center , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Qian W; a Department of Hematology , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Lin J; b Laboratory Center , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.
Qian J; b Laboratory Center , Affiliated People's Hospital of Jiangsu University , Zhenjiang , China.

Leuk Lymphoma ; 58(1): 96-103, 2017 01.

Article en En | MEDLINE | ID: mdl-27244225

RESUMEN

Abnormal methylation of let-7a-3 has been found in various cancers and may consequently affect their survival. In this study, real-time quantitative methylation specific PCR (RQ-MSP) was used to determine the unmethylation level of let-7a-3 in 95 patients with myelodysplastic syndrome (MDS). The hypomethylation of let-7a-3 promoter was detected in 22 of 95 (23.2%) patients with MDS compared to 4.2% (1/24) of controls (p= 0.0419). Moreover, the frequency of let-7a-3 hypomethylation was higher in older patients (≥70 years) than in younger patients (<70 years). No significant difference was observed in distribution of WHO, IPSS, and cytogenetic classification. However, hypomethylated patients had significantly shorter overall survival than those without hypomethylation (p= 0.007). Moreover both Kaplan-Meier and Multivariate Cox analyses confirmed that let-7a-3 hypomethylation was an independent prognostic risk factor in cohorts of MDS patients with lower-risk disease. Our study suggested that let-7a-3 hypomethylation may predict poor outcome in MDS.

Asunto(s)

Metilación de ADN; Regulación de la Expresión Génica; MicroARNs/genética; Síndromes Mielodisplásicos/genética; Síndromes Mielodisplásicos/mortalidad; Interferencia de ARN; Adulto; Anciano; Anciano de 80 o más Años; Biomarcadores; Estudios de Casos y Controles; Islas de CpG; Femenino; Humanos; Estimación de Kaplan-Meier; Masculino; Persona de Mediana Edad; Síndromes Mielodisplásicos/diagnóstico; Pronóstico; Modelos de Riesgos Proporcionales; Adulto Joven

Palabras clave

Hypomethylation; let-7a-3; myelodysplastic syndrome; prognosis

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Síndromes Mielodisplásicos / Regulación de la Expresión Génica / Metilación de ADN / MicroARNs / Interferencia de ARN Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies / Risk_factors_studies Límite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Leuk Lymphoma Asunto de la revista: HEMATOLOGIA / NEOPLASIAS Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos

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