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Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections.
Branavan, Manoharanehru; Mackay, Ruth E; Craw, Pascal; Naveenathayalan, Angel; Ahern, Jeremy C; Sivanesan, Tulasi; Hudson, Chris; Stead, Thomas; Kremer, Jessica; Garg, Neha; Baker, Mark; Sadiq, Syed T; Balachandran, Wamadeva.
Afiliación
  • Branavan M; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Mackay RE; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK. Electronic address: eesrrem@brunel.ac.uk.
  • Craw P; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK; Oceans and Atmosphere Flagship, Commonwealth Science and Industrial Research Organisation (CSIRO), Hobart, Tasmania 7001, Australia.
  • Naveenathayalan A; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Ahern JC; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Sivanesan T; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Hudson C; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Stead T; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Kremer J; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Garg N; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Baker M; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
  • Sadiq ST; Institute for Infection and Immunity, St George's University of London, Cranmer Terrace, London SW17 0RE, UK.
  • Balachandran W; College of Engineering, Design and Physical Sciences, Brunel University London, Kingston Lane, Uxbridge, UK.
Med Eng Phys ; 38(8): 741-8, 2016 08.
Article en En | MEDLINE | ID: mdl-27238759
This paper presents the design of a modular point of care test platform that integrates a proprietary sample collection device directly with a microfluidic cartridge. Cell lysis, within the cartridge, is conducted using a chemical method and nucleic acid purification is done on an activated cellulose membrane. The microfluidic device incorporates passive mixing of the lysis-binding buffers and sample using a serpentine channel. Results have shown extraction efficiencies for this new membrane of 69% and 57% compared to the commercial Qiagen extraction method of 85% and 59.4% for 0.1ng/µL and 100ng/µL salmon sperm DNA respectively spiked in phosphate buffered solution. Extraction experiments using the serpentine passive mixer cartridges incorporating lysis and nucleic acid purification showed extraction efficiency around 80% of the commercial Qiagen kit. Isothermal amplification was conducted using thermophillic helicase dependant amplification and recombinase polymerase amplification. A low cost benchtop real-time isothermal amplification platform has been developed capable of running six amplifications simultaneously. Results show that the platform is capable of detecting 1.32×10(6) of sample DNA through thermophillic helicase dependant amplification and 1×10(5) copy numbers Chlamydia trachomatis genomic DNA within 10min through recombinase polymerase nucleic acid amplification tests.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de Transmisión Sexual / Sistemas de Atención de Punto / Técnicas de Diagnóstico Molecular Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Med Eng Phys Asunto de la revista: BIOFISICA / ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de Transmisión Sexual / Sistemas de Atención de Punto / Técnicas de Diagnóstico Molecular Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Med Eng Phys Asunto de la revista: BIOFISICA / ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido