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Inhibition of Ovarian Tumor Growth by Targeting the HU177 Cryptic Collagen Epitope.
Caron, Jennifer M; Ames, Jacquelyn J; Contois, Liangru; Liebes, Leonard; Friesel, Robert; Muggia, Franco; Vary, Calvin P H; Oxburgh, Leif; Brooks, Peter C.
Afiliación
  • Caron JM; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Ames JJ; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Contois L; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Liebes L; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Friesel R; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Muggia F; New York University Langone Medical Center, Division of Hematology and Medical Oncology, New York, New York.
  • Vary CP; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Oxburgh L; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine.
  • Brooks PC; Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine. Electronic address: brookp1@mmc.org.
Am J Pathol ; 186(6): 1649-61, 2016 06.
Article en En | MEDLINE | ID: mdl-27216148
Evidence suggests that stromal cells play critical roles in tumor growth. Uncovering new mechanisms that control stromal cell behavior and their accumulation within tumors may lead to development of more effective treatments. We provide evidence that the HU177 cryptic collagen epitope is selectively generated within human ovarian carcinomas and this collagen epitope plays a role in SKOV-3 ovarian tumor growth in vivo. The ability of the HU177 epitope to regulate SKOV-3 tumor growth depends in part on its ability to modulate stromal cell behavior because targeting this epitope inhibited angiogenesis and, surprisingly, the accumulation of α-smooth muscle actin-expressing stromal cells. Integrin α10ß1 can serve as a receptor for the HU177 epitope in α-smooth muscle actin-expressing stromal cells and subsequently regulates Erk-dependent migration. These findings are consistent with a mechanism by which the generation of the HU177 collagen epitope provides a previously unrecognized α10ß1 ligand that selectively governs angiogenesis and the accumulation of stromal cells, which in turn secrete protumorigenic factors that contribute to ovarian tumor growth. Our findings provide a new mechanistic understanding into the roles by which the HU177 epitope regulates ovarian tumor growth and provide new insight into the clinical results from a phase 1 human clinical study of the monoclonal antibody D93/TRC093 in patients with advanced malignant tumors.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Colágeno / Proliferación Celular / Microambiente Tumoral / Epítopos Límite: Animals / Female / Humans Idioma: En Revista: Am J Pathol Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Colágeno / Proliferación Celular / Microambiente Tumoral / Epítopos Límite: Animals / Female / Humans Idioma: En Revista: Am J Pathol Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos