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A Rapid Method for Refolding Cell Surface Receptors and Ligands.
Zhai, Lu; Wu, Ling; Li, Feng; Burnham, Robert S; Pizarro, Juan C; Xu, Bin.
Afiliación
  • Zhai L; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
  • Wu L; Interdepartmental Microbiology Graduate Program, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
  • Li F; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
  • Burnham RS; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
  • Pizarro JC; Department of Biochemistry, Virginia Polytechnic Institute &State University, Blacksburg, VA 24061, USA.
  • Xu B; Department of Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
Sci Rep ; 6: 26482, 2016 05 24.
Article en En | MEDLINE | ID: mdl-27215173
Production of membrane-associated cell surface receptors and their ligands is often a cumbersome, expensive, and time-consuming process that limits detailed structural and functional characterization of this important class of proteins. Here we report a rapid method for refolding inclusion-body-based, recombinant cell surface receptors and ligands in one day, a speed equivalent to that of soluble protein production. This method efficiently couples modular on-column immobilized metal ion affinity purification and solid-phase protein refolding. We demonstrated the general utility of this method for producing multiple functionally active immunoreceptors, ligands, and viral decoys, including challenging cell surface proteins that cannot be produced using typical dialysis- or dilution-based refolding approaches.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cuerpos de Inclusión / Receptores de Superficie Celular Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cuerpos de Inclusión / Receptores de Superficie Celular Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido