The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases.
Prog Biophys Mol Biol
; 121(2): 123-30, 2016 07.
Article
en En
| MEDLINE
| ID: mdl-27210304
Since the creation of the first transgenic rabbit thirty years ago, pronuclear microinjection remained the single applied method and resulted in numerous important rabbit models of human diseases, including cardiac deficiencies, albeit with low efficiency. For additive transgenesis a novel transposon mediated method, e.g., the Sleeping Beauty transgenesis, increased the efficiency, and its application to create cardiac disease models is expected in the near future. The targeted genome engineering nuclease family, e.g., the zink finger nuclease (ZFN), the transcription activator-like effector nuclease (TALEN) and the newest, clustered regularly interspaced short palindromic repeats (CRISPR) with the CRISPR associated effector protein (CAS), revolutionized the non-mouse transgenesis. The latest gene-targeting technology, the CRISPR/CAS system, was proven to be efficient in rabbit to create multi-gene knockout models. In the future, the number of tailor-made rabbit models produced with one of the above mentioned methods is expected to exponentially increase and to provide adequate models of heart diseases.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas de Transferencia de Gen
/
Cardiopatías
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Prog Biophys Mol Biol
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Reino Unido