Determination of trans-phylloquinone in children's serum.
Clin Chem
; 35(5): 874-8, 1989 May.
Article
en En
| MEDLINE
| ID: mdl-2720986
By optimizing the conditions for determining trans-phylloquinone and its metabolite, K-2,3-epoxide, in serum through a two-step HPLC process combined with fluorometric detection after coulometric reduction, we have been able to develop a method applicable to small volumes of serum (200 to 500 microL). The limit of detection (signal-to-noise ratio of 3) was 15 ng/L for trans-phylloquinone, 30 ng/L for K-2,3-epoxide. The trans-phylloquinone concentrations measured by this method in serum from 82 children, ages one to six years, whose results were normal for overall coagulation tests, ranged from 40 to 880 ng/L (median 175 ng/L). We discuss these findings and compare them with vitamin K1(20) values reported for adults.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Vitamina K 1
Límite:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
1989
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido