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Intracellular chromobody delivery by mesoporous silica nanoparticles for antigen targeting and visualization in real time.
Chiu, Hsin-Yi; Deng, Wen; Engelke, Hanna; Helma, Jonas; Leonhardt, Heinrich; Bein, Thomas.
Afiliación
  • Chiu HY; Department of Chemistry and Center for NanoScience (CeNS), LMU Munich, Butenandtstrasse 5-13 (E), 81377 Munich, Germany.
  • Deng W; Department of Biology II and Center for NanoScience (CeNS), LMU Munich, Grosshadernerstrasse 2, 82152 Planegg-Martinsried, Germany.
  • Engelke H; Department of Chemistry and Center for NanoScience (CeNS), LMU Munich, Butenandtstrasse 5-13 (E), 81377 Munich, Germany.
  • Helma J; Department of Biology II and Center for NanoScience (CeNS), LMU Munich, Grosshadernerstrasse 2, 82152 Planegg-Martinsried, Germany.
  • Leonhardt H; Department of Biology II and Center for NanoScience (CeNS), LMU Munich, Grosshadernerstrasse 2, 82152 Planegg-Martinsried, Germany.
  • Bein T; Department of Chemistry and Center for NanoScience (CeNS), LMU Munich, Butenandtstrasse 5-13 (E), 81377 Munich, Germany.
Sci Rep ; 6: 25019, 2016 05 13.
Article en En | MEDLINE | ID: mdl-27173765
Chromobodies have recently drawn great attention as bioimaging nanotools. They offer high antigen binding specificity and affinity comparable to conventional antibodies, but much smaller size and higher stability. Chromobodies can be used in live cell imaging for specific spatio-temporal visualization of cellular processes. To date, functional application of chromobodies requires lengthy genetic manipulation of the target cell. Here, we develop multifunctional large-pore mesoporous silica nanoparticles (MSNs) as nanocarriers to directly transport chromobodies into living cells for antigen-visualization in real time. The multifunctional large-pore MSNs feature high loading capacity for chromobodies, and are efficiently taken up by cells. By functionalizing the internal MSN surface with nitrilotriacetic acid-metal ion complexes, we can control the release of His6-tagged chromobodies from MSNs in acidified endosomes and observe successful chromobody-antigen binding in the cytosol. Hence, by combining the two nanotools, chromobodies and MSNs, we establish a new powerful approach for chromobody applications in living cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Coloración y Etiquetado / Técnicas Citológicas / Dióxido de Silicio / Nanopartículas / Imagen Óptica / Anticuerpos / Antígenos Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Coloración y Etiquetado / Técnicas Citológicas / Dióxido de Silicio / Nanopartículas / Imagen Óptica / Anticuerpos / Antígenos Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido