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Characterization of grass carp spleen transcriptome during GCRV infection.
Xu, B H; Zhong, L; Liu, Q L; Xiao, T Y; Su, J M; Chen, K J; Wang, H Q; Dai, Y J; Chen, J.
Afiliación
  • Xu BH; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Zhong L; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Liu QL; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Xiao TY; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Su JM; College of Animal Veterinary and Medicine, Hunan Agricultural University, Changsha, China.
  • Chen KJ; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Wang HQ; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Dai YJ; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
  • Chen J; College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
Genet Mol Res ; 15(2)2016 Apr 25.
Article en En | MEDLINE | ID: mdl-27173223
The aim of the study was to investigate the grass carp hemorrhagic infection pathway and its key-related genes. Grass carp reovirus (GCRV) might cause hemorrhagic disease in grass carps. Healthy grass carp fingerlings (N = 60) were divided into control and infected groups. Fish in the control group were intraperitoneally (ip) injected with 0.6% fish physiological saline; the infected group received 5,000,000 50% tissue culture infective doses of GCRV 873 standard strain, a double-stranded RNA (dsRNA) virus strain, ip, in 0.5 mL. Illumina HiSeqTM 2000 was used for transcriptome sequencing, and real-time polymerase chain reaction (PCR) used to detect complement factors II (C2), III (C3), and V (C5); profibrinolysin (PLG); and coagulation factor II (F2) expression. A total of 2,722,223 reads were detected in the control group, and 2,751,111 in the infected group. Among 11,023 unigenes obtained after transcriptome assembly, 10,021 unigenes were significantly differentially expressed. Gene ontology and KEGG analysis, a collection of databases dealing with genomes and biological pathways, were performed to classify unigenes into functional categories, to understand gene function and identify regulatory pathways. Real-time PCR analysis showed that C2, C3, C5, PLG, and F2 expression levels were down-regulated, confirming results of pathway-enrichment analysis. This is the first application of high-throughput sequencing technology to investigate the in vivo effects of GCRV, on genes and pathways involved in the immune response to infection in grass carp.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bazo / Carpas / Infecciones por Reoviridae / Transcriptoma Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Brasil
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bazo / Carpas / Infecciones por Reoviridae / Transcriptoma Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Genet Mol Res Asunto de la revista: BIOLOGIA MOLECULAR / GENETICA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Brasil